Transgenic Production Service (TPS)

The Transgenic Production Service (TPS) provides a resource for investigators seeking to generate transgenic and gene-targeted mice for their research activities. The facility also manages the IVIS Spectrum in vivo imaging system, used for the quantitative monitoring of bioluminescent and fluorescent reporters in living mice.

Overview

Our services include pronuclear microinjection of DNA transgenes into mouse zygotes for the production of transgenic founders; gene targeting in ES cells; microinjection of targeted ES cell lines into blastocysts to produce chimeric mice; cryopreservation of embryos and sperm; and recovery of live mice from cryopreserved embryos and sperm. Training and assistance on the IVIS imaging system is also provided.

Investigators developing grant proposals that include the use of engineered animals are invited to contact the TPS Director for help with experimental design and for assistance in preparing proposed budgets for transgenic/gene-targeted mouse production. We are happy to provide you with a letter stating our readiness to make transgenic and/or chimeric mice for submission to funding agencies.

Services:

  • DNA Microinjection/Transgenics
  • ES Cell Microinjection/Chimeric mice
  • ES cell expansion
  • Gene Targeting in ES cells
  • Embryo and Sperm Cryopreservation
  • Recovery of Pups from Cryopreserved Embryos
  • Recovery of Pups from Cryopreserved Sperm - In Vitro Fertilization (IVF)
  • IVIS Imaging