Surface Plasmon Resonance (SPR)
Surface Plasmon Resonance (SPR) facility at RRC UIC provides access to very advanced SPR instrument Biacore T200 on pay-per-use basis. SPR technology can monitor real time interaction between two molecules at high sensitivity. Hand-on training is available for long term users, and assisted runs by an expert are also offered for investigators who occasionally need to do experiments.
RRC Surface Plasmon Resonance (SPR) facility offers the real-time detection and monitoring of the biomolecule interactions such as proteins-proteins, proteins-peptides, proteins-DNA, and proteins-compounds. Our Biacore T200 can detect from large protein complexes (> 100 kDa) to a very small fragment compounds (< 100 Da), and it can provide quantitative information on binding specificity, binding affinity, kinetics, and concentration. This technique can also be applied to every stage of drug-discovery, which includes mid-throughput automated compound screening, hit confirmation and validation, hit characterization via kinetics, and mechanism of action.