Mass spectrometry, Metabolomics & Proteomics Facility (MMPF)

The Mass spectrometry, Metabolomics & Proteomics Facility (MMPF) serves as a campus-wide research core facility and a resource for the analysis of a wide variety of molecules ranging from low molecular weight volatile compounds to proteins. We provide open-access, for trained users, to a majority of our instrumentation and also can provide full service mass spectrometry, metabolomics and proteomics.

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MMPF provides many types of mass spectrometry measurements include qualitative and quantitative analysis, purity assessment, molecular weight determination, and high-resolution exact mass measurements for the determination of elemental compositions. Using liquid chromatography mass spectrometry (LC-MS) and gas chromatography mass spectrometry (GC-MS) we can analyze the composition of complex mixtures. Tandem mass spectrometry (MS-MS) provides information regarding molecular structure of organic compounds.

MMPF also provides investigators access to a number of instruments optimized for use in proteomics, metabolomics, biomarker discovery and other quantitative studies. Scientists in our facility can design and conduct experimental protocols, analyze data and provide pre-experimental consultation to investigators who seek to incorporate mass spectrometry based experimental strategies into their research programs. Researchers may submit samples to MMPF for protein identification, post-translational modification identification, quantitative proteomics, molecular weight determination and data analysis. Alternatively, qualified users may schedule instrument time to analyze their own samples. We utilize Mascot, MassMatrix, SEQUEST and specialty search engine software to identify proteins and post-translational modifications in your samples. Our software tools are available for self-serve users to analyze their own data on workstations in the main mass spectrometry lab and the proteomics office.

Currently fourteen instruments are in use: a high resolution hybrid line ion trap-OrbiTrap Velos LC-MS with ETD, a high resolution hybrid linear ion trap-Fourier Transform ICR LC-MS with ECD, a high resolution q-TOF tandem LC-MS with ion mobility, a high resolution IT-TOF LC-MS, a high resolution MALDI-TOF, a high resolution MALDI-TOF/TOF, a single quadrupole LC-MS, a single quadrupole SFC-MS, three triple quadrupole LC-MS, a quadrupole ion trap LC-MS, a linear ion trap LC-MS, and a high resolution magnetic sector GC-MS.

There are two Mass Spectrometry laboratories, one in 550, College of Pharmacy Building, 833 South Wood Street, and the other in Room 109A, Science and Engineering South Building, 845 West Taylor Street. The Proteomics office is located in E-125, Medical Sciences Building, 835 South Wolcott Ave.

The Proteomics Service of MMPF was established in part by a grant from The Searle Funds at The Chicago Community Trust to the Chicago Biomedical Consortium (