Xenogen (Caliper Life Sciences) IVIS Spectrum

Xenogen (Caliper Life Sciences) IVIS Spectrum

Location: Biologic Resources Center (BRL), 112

;
  Unassisted Assisted
(1/2 hour Minimum)
UIC Cost $26.00 (0.5hr) $46.50 (0.5hr)
Other Academic $inquire (0.5hr) $inquire (0.5hr)
Non-Academic$inquire (0.5hr) $inquire (0.5hr)

Description

FOR MORE INFORMATION ON THE SPECTRUM'S FEATURES AND IMAGING APPLICATIONS visit http://www.perkinelmer.com/Catalog/Category/ID/In%20Vivo%20Imaging/. Caliper Life Sciences is now part of PERKINELMER.

Also visit our wiki page at http://wiki.rrc.uic.edu/wiki/RRC-TPS:_Xenogen_IVIS_Spectrum.

The Xenogen IVIS® Spectrum in vivo imaging system from Caliper Life Sciences uses Xenogen’s novel patented optical imaging technology to facilitate non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals. Quantitative monitoring of both bioluminescent and fluorescent probes and reporters across the blue to almost infrared wavelength spectrum is possible. NOTE: GFP is not recommended for most fluorescent imaging applications; alternatively, plan to use a red shifted fluorescent protein such as cherry, tomato, or katushka. The Spectrum is equipped with a gas anesthesia unit that allows continuous administration of isoflurane gas for anesthetizing animals during imaging. The system’s Living Image 4.0 image acquisition and analysis software includes features for spectral unmixing to remove unwanted autofluorescence and to observe and separate several fluorescent and bioluminescent reporters within the same animal. It can also create 3D tomography images that permit source localization and concentration, as well as anatomical analysis using a 3D mouse atlas.

FEATURES

  • High-sensitivity in vivo imaging of fluorescence and bioluminescence
  • High throughput (5 mice) with 23 cm field of view
  • High resolution (to 20 microns) with 3.9 cm field of view
  • Twenty eight high efficiency filters spanning 430 – 850 nm - Supports spectral unmixing applications - Ideal for distinguishing multiple bioluminescent and fluorescent reporters
  • Optical switch in the fluorescence illumination path allows reflection-mode or transmission-mode illumination
  • 3D diffuse tomographic reconstruction for both fluorescence and bioluminescence that can be analyzed in an anatomical context using The Digital Mouse Atlas.
  • Ability to import and automatically co-register CT or MRI images yielding a functional and anatomical context for your scientific data.
  • NIST traceable absolute calibrations
  • Gas anesthesia inlet and outlet ports

The instrument is equipped with the XGI-8 Gas Anesthesia Module, which integrates efficient gas delivery while minimizing excess gas exposure to lab personnel into a single system. The Gas Anesthesia System delivers isoflurane gas to a 5-port anesthesia manifold (for work with mice) housed in the IVIS System imaging chamber.

Technical Specifications

Power Requirements 20 Amps for 120 VAC or 10 Amps for 230 VAC Dimensions •Imaging Chamber Interior Size: 43 x 50 x 60 cm (W x D x H) •Imaging System Space Requirement: 203 x 163 x 214 cm (W x D x H) Camera Sensor Back-thinned, back-illuminated Grade 1 CCD CCD Size 2.7 x 2.7 cm Operating Temperature -90°C Imaging Pixels 2048 x 2048 Quantum Efficiency >85% 500 – 700 nm; >30% 400 – 900nm Pixel Size 13.5 microns Minimum Detectable Radiance 70 photons/s/sr/cm2 Field of View (FOV) Minimum FOV 3.9 x 3.9 cm Maximum FOV 23 x 23 cm Minimum Image Pixel Resolution 20 microns Read Noise < 3 electrons for bin=1,2,4; < 5 electrons for bin=8,16 Dark Current (Typical) <100 electrons/s/cm2 Filter Slots Fluorescence Excitation 12 Fluorescence Emission 24 Fluorescence Filters Excitation 10 Emission 18 Heated Chamber Yes Diffuse Tomography Software Yes Gas Anesthesia Yes Computer 2.8 GHz, 1 GB RAM, RW CD/DVD, 80 GB HD, 20” flat screen Lens f/1 – f/8, 1.5x, 2.5x, 5x, 8.7x magnifications Stage Temperature 20 – 40°C Transillumination Stage Yes Workbench Yes