Carl Zeiss LSM 510

Carl Zeiss LSM 510

Location: Medical Sciences Building, E-32D

;
  Unassisted Assisted
(1 hr)
UIC Cost $35 (per hour) $70 (per hour)
Other Academic $55 (per hour) $109 (per hour)
Non-Academic$105 (per hour) $210 (per hour)

Description

The LSM 510 was decommissioned in 2011 after over 10 years of regular use. Several of its excellent objectives were used to complete the installation of an upgraded Zeiss LSM 710 META system. However, a number of remaining components are still being housed by the Confocal Microscopy Facility. If you are a UIC investigator and might find some of these components useful, please contact the CMF staff.

Technical Specifications

Available excitation lasers include:
• 80 mW UV laser = 351, 364 nm
• 25 mW KrAr laser = 458, 568 nm
• 5 mW HeNe laser = 633 nm

The "Ch1" PMT1 emission filter options have the following designations:
• LP 385 (DAPI)
• LP 505 (GFP, FITC, Alexa 488)
• BP 505-550 (GFP, FITC, Alexa 488)
• LP 590 (CY3, Rhod, TxRd)
• BP 585-615 (CY3, Rhod, TxRd)
• LP 650 (CY5)
• BP 470-500 (INDO)

The "Ch2" PMT2 emission filter options have the following designations:
• BP 385-470 (DAPI)
• BP 350-430 (INDO)
• LP 505 (GFP, FITC, Alexa 488)
• BP 500-530 (GFP, FITC, Alexa 488)
• BP 500-550 (GFP, FITC, Alexa 488)
• BP 585-615 (CY3, Rhod, TxRd)

The "Ch3" PMT3 emission filter options have the following designations:
• LP 505 (GFP, FITC, Alexa 488)
• BP 505-530 (GFP, FITC, Alexa 488)
• BP 505-550 (GFP, FITC, Alexa 488)
• BP 585-615 (CY3, Rhod, TxRd)

The "Ch4" PMT4 emission filter options have the following designations:
• BP 585-615 (CY3, Rhod, TxRd)