2009-01-20T10:41:40-06:00 SDS gel 10 51 2 2D SDS PAGE 300 N/A 225 N/A 2 200 N/A 2009-02-02T14:39:20-06:00 2009-06-15T17:08:32-05:00 Assisted / Unassisted 3 82 1 350 MHz NMR 116 16 50 8 1 35 5 2009-06-15T17:08:32-05:00 2009-06-15T17:04:01-05:00 Unassisted / Assisted 3 81 1 500 MHz NMR 150 25 75 15 1 50 10 2009-06-15T17:04:01-05:00 2009-06-23T10:51:46-05:00 Use of the ABI Voyager DE-PRO. Billing is done through the Proteomics & Informatics Services Facility - please visit their web site at http://proteomics.rrc.uic.edu/ and click on Service Price List for more details/ options. 1 83 1 ABI Voyager DE-PRO - see Proteomics website na na na na 1 na na 2009-06-24T09:41:54-05:00 2008-12-05T08:14:37-06:00 Additional training is charged at the assisted hourly rate. This training can be either conversion training to a different instrument, or training to use an advanced technique such as XEDS, EELS or STEM. 2 14 1 Additional Training 236 na 119 na 1 76 na 2008-12-05T08:14:37-06:00 2009-07-21T18:27:27-05:00 . 7 107 1 Additional training for real time PCR (above and beyond normal training) 40 - 40 - 1 40 - 2009-07-21T18:27:27-05:00 2009-07-21T11:51:28-05:00 - 6 89 2 Affymetrix Service: One-cycle RNA labeling - - - - 2 245 - 2009-10-30T14:08:43-05:00 2009-10-30T14:10:29-05:00 - 6 111 2 Affymetrix service: ST array labeling - - - - 2 280 - 2009-10-30T14:10:29-05:00 2009-07-21T11:54:11-05:00 GeneChip Hybridization 6 91 2 Affymetrix: GeneChip Hybridization - - - - 2 75 - 2009-10-30T14:21:44-05:00 2009-01-22T10:54:47-06:00 Analysis of stand-alone FCS Mac/PC 8 73 1 Analysis On Stand-alone FCS Mac/PC na 19 na 9 1 na 6 2009-01-22T11:01:46-06:00 2009-07-21T18:20:47-05:00 . 7 99 2 Analysis: user provides dye-terminator sequence reactions 6 - 5 - 2 4 - 2009-07-21T18:20:47-05:00 2009-01-22T10:56:05-06:00 AutoMACS 8 74 1 AutoMACS na 40 na 20 12 na 13 2009-01-22T10:56:05-06:00 2009-07-21T18:22:56-05:00 . 7 101 2 BAC/YAC Sequencing and Analysis: user provides DNA template 10.50 - 8.50 - 2 5.50 - 2009-07-21T18:22:56-05:00 2008-12-05T08:07:36-06:00 Includes basic operational training over a period of 10 hours which must be completed in a 30 day period. Initial training will use a well characterized specimen supplied by EMS staff. Later on during the training period project specific specimens can be used. 2 13 1 Basic Training on TEM or SEM 2040 na 942 na 3 608 na 2008-12-05T08:07:36-06:00 2009-01-20T10:54:47-06:00 Gel Scan 10 57 2 BioRad Scanner (30 Minutes) N/A 20 N/A 15 3 N/A 10 2009-01-20T10:54:47-06:00 2009-01-22T11:06:25-06:00 Cell Counter 8 75 1 Cell Counter na 4 na 3 14 na 2 2009-01-26T08:56:59-06:00 2009-07-21T18:25:01-05:00 . 7 104 2 Chromatogram printouts 4 - 3 - 2 2 - 2009-07-21T18:25:01-05:00 2009-01-20T10:53:03-06:00 CD spectra 10 56 2 Circular Dichroism Spectrometer 30 30 20 20 2 15 20 2009-01-20T10:53:03-06:00 2009-07-21T18:23:38-05:00 . 7 102 2 Clean PCR reaction 5 - 4 - 2 3 - 2009-07-21T18:23:38-05:00 2009-01-22T10:36:30-06:00 Cloning setup 8 69 1 Cloning setup 93 na 47 na 12 30 na 2009-01-22T10:36:30-06:00 2009-01-22T10:30:03-06:00 Cyan 8 67 1 Cyan 170 102 85 51 1 55 33 2009-01-22T10:30:03-06:00 2009-06-25T15:39:56-05:00 Cytospin 3 5 86 1 Cytospin 3 - 24 - 12 1 - 8 2009-06-25T15:39:56-05:00 2009-07-21T12:07:29-05:00 - 6 98 2 Data acquisition/analysis - - - - 1 50 - 2009-10-30T14:25:57-05:00 2009-01-22T10:53:27-06:00 Data analysis 8 72 1 Data Analysis 170 53 85 26 1 55 17 2009-01-22T11:00:31-06:00 2009-01-16T10:43:18-06:00 Will Design,Engineer,and/or Fabricate any device needed 14 49 2 Design/Engineer/Fabricate NA 96 NA 44 1 NA 38 2009-01-16T10:45:47-06:00 2009-01-06T17:14:50-06:00 The Production Fee (actual charge to PIs RRC account) is $1,825. The fee shown is the estimated total charge, which also includes mouse purchase & shipping costs and per diem. These additional costs are charged directly to the PIs account at the BRL. The BRL may also request mouse pathogen screening for animals moved to certain housing areas on campus. These tests are also charged directly to the BRL account. 11 28 2 DNA Microinjection: C57BL6 strain inquire - inquire - 5 3,425 - 2009-06-04T14:41:49-05:00 2008-12-27T19:44:48-06:00 The Production Fee (actual charge to PIs RRC account) is $1,725. The fee shown is the estimated total charge, which also includes mouse purchase & shipping costs and per diem. These additional costs are charged directly to the PIs account at the BRL. The BRL may also request mouse pathogen screening for animals moved to certain housing areas on campus. These tests are also charged directly to the BRL account. 11 27 2 DNA Microinjection: FVBN strain inquire - inquire - 5 3,325 - 2009-06-04T14:40:58-05:00 2008-12-27T19:26:36-06:00 The Production Fee (actual charge to PIs RRC account) is $1,725. The fee shown is the estimated total charge, which also includes mouse purchase & shipping costs and per diem. These additional costs are charged directly to the PIs account at the BRL. The BRL may also request mouse pathogen screening for animals moved to certain housing areas on campus. These tests are also charged directly to the BRL account. 11 26 2 DNA Microinjection: outbred strain inquire - inquire - 5 2,725 - 2009-06-04T14:29:22-05:00 2008-12-05T08:16:45-06:00 Use of the following electron microscopes JEOL JEM-1220, JEOL JEM-2010F, JEOL JEM-3010, JEOL JEM-100CX, Hitachi S-3000N, JEOL JSM-6320F 2 15 1 Electron Microscopes 236 159 119 60 1 76 38 2008-12-05T08:20:39-06:00 2009-01-22T10:27:42-06:00 Elite 8 65 1 Elite 170 102 85 51 1 55 33 2009-01-22T10:27:42-06:00 2009-01-06T17:44:17-06:00 The Production Fee (actual charge to PIs RRC account) is $540. The fee shown is the estimated total charge, which also includes mouse purchase & shipping and per diem. These additional costs are charged directly to the PIs account at the BRL. The cost for outbred strains will be lower. 11 33 2 Embryo Cryopreservation - - - - 8 775 - 2009-06-04T14:45:17-05:00 2009-01-06T17:53:13-06:00 The Production Fee (actual charge to PIs RRC account) is $855. The fee shown is the estimated total charge, which also includes mouse purchase & shipping and per diem. These additional costs are charged directly to the PIs account at the BRL. The cost for outbred strains will be lower. 11 34 2 Embryo Cryopreservation (w/ test thaw) - - - - 8 1,265 - 2009-06-04T14:45:48-05:00 2009-01-06T17:19:21-06:00 The Production Fee (actual charge to PIs RRC account) is $1,725. The fee shown is the estimated total charge, which also includes mouse purchase & shipping costs and per diem. These additional costs are charged directly to the PIs account at the BRL. The BRL may also request mouse pathogen screening for animals moved to certain housing areas on campus. These tests are also charged directly to the BRL account. Note: 3-4 ES clones are typically injected in 3 sessions. Cells must be tested for mycoplasma and mouse parvo virus prior to submission for microinjection. 11 29 2 ES Cell Microinjection inquire - inquire - 6 3,305 - 2009-06-04T14:42:40-05:00 2009-01-06T17:25:11-06:00 The Production Fee (actual charge to PIs RRC account) is $1,125. The fee shown is the estimated total charge, which also includes mouse purchase & shipping costs and per diem. These additional costs are charged directly to the PIs account at the BRL. The BRL may also request mouse pathogen screening for animals moved to certain housing areas on campus. These tests are also charged directly to the BRL account. Note: 1-2 ES clones are typically injected in 2 sessions. Cells must be tested for mycoplasma and mouse parvo virus prior to submission for microinjection. 11 30 2 ES Cell Microinjection inquire - inquire - 7 2,425 - 2009-06-04T14:43:13-05:00 2008-12-05T08:42:19-06:00 Charge for exact mass analysis when no low resolution spectrum has been acquired or submitted. If exact mass is not possible user will be charged for, and given, a low resolution spectrum. 1 22 2 Exact Mass Analysis (Assisted) inquire na 109 na 2 53 na 2009-10-19T17:25:25-05:00 2008-06-25T11:37:01-05:00 Additional charge for exact mass analysis after collection of a low resolution spectrum or submission of a low resolution spectrum with the sample 1 4 2 Exact Mass Analysis (Assisted): after low-resolution spectrum inquire na 73 na 2 35 na 2009-10-19T17:24:42-05:00 2009-01-08T16:03:08-06:00 Includes freezing up to 5 vials of cells (additional vials at $10 each) and mycoplasma testing after expansion. Note: Cells must have been tested for mycoplasma prior to submission for expansion. 11 42 2 Expansion of ES Cells Propagated Outside TPS inquire - inquire - 10 280 - 2009-06-04T14:49:07-05:00 2009-01-07T15:34:28-06:00 Includes: Electroporation (1 clone) with Cre or Flp vector; Picking, propagation & freezing clones (96); DNA prep for genotyping (investigator performs genotyping); Expansion & freezing (4)* positive clones; DNA prep from positive clones for genotype confirmation (investigator performs genotyping); Metaphase spreads, chromosome counts; Mycoplasma testing. *Additional clones at $630 per clone. 11 41 2 Expression of Cre Recombinase in ES Cells inquire - inquire - 10 2,170 - 2009-06-08T14:28:49-05:00 2009-01-22T10:40:32-06:00 FACsCalibur 8 71 1 FACsCalibur na 68 na 34 1 na 22 2009-01-22T10:40:32-06:00 2009-01-22T10:23:27-06:00 Full service after hours 8 63 1 Full service After Hours 212.50 na 106.25 na 1 68.75 na 2009-01-22T10:57:39-06:00 2009-01-15T14:50:22-06:00 Unlimited use of our GCG server for a low monthly subscription. Subscriptions are auto-renewing until canceled (please e-mail gcg@mail.rrc.uic.edu for additional assistance). You may cancel your subscription at any time, but we are unable to refund any unused portion of the monthly fee. 12 48 3 GCG SeqLab NA 25 NA 25 11 NA 25 2009-01-15T14:53:24-06:00 2009-01-07T15:08:50-06:00 Includes: Electroporation & drug selection; Picking, propagation & freezing drug-selected clones (four 96-well plates); DNA prep for genotyping (investigator performs genotyping); Expansion & freezing (4)* positive clones; DNA prep from positive clones for genotype confirmation (investigator performs genotyping); Metaphase spreads, chromosome counts; Mycoplasma testing. *Additional clones at $630 per clone. 11 40 2 Gene Targeting in ES Cells inquire - inquire - 9 6,130 - 2009-06-08T14:25:47-05:00 2009-07-21T11:58:29-05:00 Hybridization 6 94 2 Glass Array: Hybridization - - - - 2 25 - 2009-10-30T14:21:04-05:00 2009-07-21T11:55:55-05:00 - 6 92 2 Glass Array: RNA labeling - - - - 2 120 - 2009-10-30T14:20:15-05:00 2009-07-21T11:59:50-05:00 Scanning - training/assisted 6 95 2 Glass Array: Slide scanning - - - - 2 50 - 2009-10-30T14:28:29-05:00 2009-01-20T10:47:30-06:00 Protein Purification 10 53 2 HPLC purification 300 N/A 250 N/A 2 200 N/A 2009-02-02T14:40:02-06:00 2008-12-05T08:51:52-06:00 These charges are for access to the mass spectrometers (except Thermo LTQ-FT, Thermo LTQ and Voyager DE; see http://proteomics.rrc.uic.edu/ for rates and scheduling of these instruments) on a self use or assisted use basis. 1 23 1 Instrument Access (except Voyager, LTQ & LTQ-FT) inquire - 81 52 1 52 25 2009-10-19T17:25:50-05:00 2009-06-04T11:01:29-05:00 . 17 78 5 instrument usage 250 200 200 150 4 150 100 2009-07-24T14:31:44-05:00 2008-12-05T08:32:21-06:00 Use of the Fischione EAF1010 or EAF3000 or the Gatab Duo-Mill for ion beam thinning of specimens 2 21 2 Ion Mill 158 105 79 20 4 51 13 2008-12-05T13:41:34-06:00 2008-07-15T14:43:41-05:00 ... 5 8 1 Live Cell System Setup 102 - 51 - 3 35 - 2009-06-25T15:38:08-05:00 2008-06-25T11:29:35-05:00 Collection of a low resolution mass spectrum 1 3 2 Low Resolution Analysis (Assisted) inquire na 36 na 2 18 na 2009-10-19T17:24:23-05:00 2009-06-25T12:42:51-05:00 LSM 510 and LSM 510 META confocal microscopes 5 84 1 LSM 510/510 META 202 102 109 55 1 70 35 2009-06-25T15:28:07-05:00 2009-01-22T10:28:54-06:00 LSR 8 66 1 LSR 170 102 85 51 1 55 33 2009-01-22T10:28:54-06:00 2009-01-08T16:22:35-06:00 Cells must be tested for mycoplasma prior to submission for chromosome counts. 11 43 2 Metaphase Spreads (Chromosome Counts) inquire - inquire - 10 380 - 2009-06-04T14:50:32-05:00 2008-07-15T14:46:12-05:00 ... 5 10 1 Microinjection System Setup 102 - 51 - 3 35 - 2009-06-25T15:38:30-05:00 2008-07-15T14:47:07-05:00 $15/hr plus cost of using the Meta 5 11 1 Microinjection System Use 264 156 132 78 1 85 50 2009-06-25T15:58:28-05:00 2009-01-22T10:18:57-06:00 MoFlo Non-sorting experiments 8 61 1 MoFlo Non-sorting Experiments 170 na 85 na 1 55 na 2009-01-22T10:56:51-06:00 2009-01-22T10:16:45-06:00 MoFlo Sort 8 60 1 MoFlo Sort 232 na 116 na 1 75 na 2009-01-22T10:46:11-06:00 2009-01-20T14:06:01-06:00 MAb 10 59 2 Monoclonal Antibody Producton 6000 N/A 5000 N/A 2 3800 N/A 2009-02-02T14:42:43-06:00 2009-01-08T16:35:05-06:00 This service is offered by the DNA Sequencing (DNAS) Facility. Please contact the TPS Director for instructions on sample preparation. 7 44 2 Mycoplasma PCR Testing inquire - inquire - 10 30 - 2009-01-15T17:37:19-06:00 2009-01-08T16:38:05-06:00 This service is offered by the DNA Sequencing (DNAS) Facility http://www.uic.edu/depts/rrc/dnas/. Please contact the TPS Director for instructions on sample preparation. 11 45 2 Mycoplasma PCR Testing inquire - inquire - 10 30 - 2009-06-04T14:50:54-05:00 2009-01-15T14:43:31-06:00 On-site service/consultation for computer repairs and upgrades. 12 47 1 On-site service/consultation NA 88 NA 58 1 NA 48 2009-01-15T14:53:15-06:00 2008-12-05T08:25:35-06:00 Use of the Veeco GEN II Oxide Molecular Beam Epitaxy system in conjunction with Prof. Ghosh (Elec Eng) or the Omicron VT SPM in conjunction with Prof. Trenery (Chemistry) 2 18 2 Oxide MBE/ UHV SPM 620 310 310 155 4 200 100 2008-12-05T13:40:16-06:00 2009-01-22T10:25:09-06:00 Penalty, unscheduled full service after hours 8 64 1 Penalty, Unscheduled Full Service after hours 155 na 78 na 12 50 na 2009-01-22T10:58:21-06:00 2009-01-20T10:49:13-06:00 Antibody Purification 10 54 2 Peptide Affinity Purification 400 N/A 280 N/A 2 260 N/A 2009-02-02T14:40:46-06:00 2009-01-20T10:35:36-06:00 Per Amino Acid 10 50 2 Peptide synthesis (per Amino acid residue) 25 N/A 22 N/A 2 20 N/A 2009-01-20T10:45:05-06:00 2009-07-21T18:28:09-05:00 . 7 108 2 Plate Sequencing 24-48 Samples 8.00 - 6.00 - 2 4.50 - 2009-07-21T18:28:09-05:00 2009-07-21T18:29:30-05:00 . 7 109 2 Plate Sequencing 48-96 Samples 7.50 - 5.50 - 2 3.50 - 2009-07-21T18:29:30-05:00 2009-01-20T14:03:54-06:00 Antipeptide Ab 10 58 2 Polyclonal Antibody Production 1200 N/A 900 N/A 2 850 N/A 2009-02-02T14:42:12-06:00 2009-01-08T16:44:18-06:00 1-2 clones are typically prepared for two microinjection sessions; 3-4 clones are typically prepared for three microinjection sessions. The cost shown applies to cells targeted and/or expanded in the TPS. Clones targeted in PIs lab are generally prepped by the PI; upon request these cells may be prepped by the TPS at $150 per clone. 11 46 2 Preparation of ES Cells for Microinjection inquire - inquire - 10 50 - 2009-06-08T14:30:47-05:00 2009-01-20T10:50:47-06:00 Protein Purification 10 55 2 Protein A/G column Purification 300 N/A 220 N/A 2 200 N/A 2009-02-02T14:41:30-06:00 2008-12-05T08:19:53-06:00 Use of the Rennishaw Ramascope 2000 2 17 1 Raman Spectroscopy 204 108 102 43 1 65.75 27.75 2008-12-05T08:19:53-06:00 2009-07-21T18:26:25-05:00 . 7 106 2 Real Time PCR - 384 well 50 - 40 - 19 30 - 2009-07-21T18:26:25-05:00 2009-07-21T18:25:41-05:00 . 7 105 2 Real Time PCR - 96 well 25 - 20 - 19 15 - 2009-07-21T18:25:41-05:00 2009-01-06T17:56:55-06:00 The Production Fee (actual charge to PIs RRC account) is $475. The fee shown is the estimated total charge, which also includes mouse purchase & shipping costs and per diem. These additional costs are charged directly to the PIs account at the BRL. The BRL may also request mouse pathogen screening for animals moved to certain housing areas on campus. These tests are also charged directly to the BRL account. 11 35 2 Recovery of Pups from Frozen Embryos inquire - inquire - 8 635 - 2009-06-04T14:44:16-05:00 2009-01-06T18:17:34-06:00 The Production Fee (actual charge to PIs RRC account) is $675. The fee shown is the estimated total charge, which also includes mouse purchase & shipping costs and per diem. These additional costs are charged directly to the PIs account at the BRL. The BRL may also request mouse pathogen screening for animals moved to certain housing areas on campus. These tests are also charged directly to the BRL account. 11 38 2 Recovery of pups from Frozen Sperm (IVF) inquire - inquire - 8 1,005 - 2009-06-04T14:49:47-05:00 2009-07-21T12:04:57-05:00 - 6 97 2 RNA purification - - - - 2 50 - 2009-10-30T14:25:17-05:00 2009-07-21T18:22:03-05:00 . 7 100 2 Sequencing & Analysis: user provides DNA template 10.50 - 8.50 - 2 5.50 - 2009-07-21T18:22:03-05:00 2009-01-22T10:35:03-06:00 Setup for sterile and non-sterile sorts 8 68 1 Sort Setup 93 na 47 na 12 30 na 2009-01-22T10:59:21-06:00 2008-12-05T08:28:41-06:00 Use of specimen preparation equipment (except ultramicrotome and ion mills) located in Room 116A, SES and Room E5B, MSB 2 19 2 Specimen Preparation Laboratory 158 105 79 20 2 51 13 2008-12-05T13:41:06-06:00 2009-02-02T14:44:37-06:00 Protein Analysis 10 76 2 Spectroscopic Analysis of Peptide or Protein Inquire N/A Inquire N/A 2 Inquire N/A 2009-02-02T14:44:37-06:00 2009-01-06T18:09:30-06:00 Cryopreserving sperm is simple and inexpensive. To recover pups at a later date, in vitro fertilization (IVF) will have to be performed. Note, even with recent improvements to procedures for sperm cryopreservation there remain differences in the success rate (% fertilization) of IVF between strains. 11 36 2 Sperm Cryopreservation - - - - 8 240 - 2009-10-12T13:37:41-05:00 2009-01-22T10:37:47-06:00 Surcharge for multi-laser experiments 8 70 1 Surcharge For Multi-laser Experiments 47 na 23 na 12 15 na 2009-01-22T11:00:02-06:00 2008-12-05T08:18:02-06:00 Use of the Kratos AXIS-165 2 16 1 Surface Analysis 236 159 119 60 1 76 38 2008-12-05T08:18:02-06:00 2009-07-21T18:24:08-05:00 . 7 103 2 Template preparation from culture for sequencing 8 - 6 - 2 4 - 2009-07-21T18:24:08-05:00 2008-12-05T09:01:52-06:00 Use of the Thermo LTQ. Billing is done through the Proteomics & Informatics Services Facilty - please visit their web site at http://proteomics.rrc.uic.edu/ and click on Service Price List for more details/ options. 1 25 1 Thermo LTQ - see Proteomics website na na na na 1 na na 2009-06-24T09:41:23-05:00 2008-12-05T08:59:57-06:00 Use of the Thermo LTQ-FT. Billing is done through the Proteomics & Informatics Services Facility - please visit their web site at http://proteomics.rrc.uic.edu/ and click on Service Price List for more details/ options. 1 24 1 Thermo LTQ-FT - see Protemics website na na na na 1 na na 2009-06-24T09:40:45-05:00 2009-06-25T15:36:28-05:00 TIRF Microscope 5 85 1 TIRF Microscope 150 75 78 39 1 50 25 2009-06-25T17:10:40-05:00 2009-06-25T17:14:00-05:00 Use of the TIRF microscope for standard widefield applications. Long-term experiments 5 days or more must include 2 days on the weekend. 5 88 1 TIRF Microscope- widefield 94 46 47 23 1 30 15 2009-06-25T17:14:00-05:00 2008-07-15T14:41:46-05:00 ... 5 7 1 Training (4hrs) 812 - 406 - 3 280 - 2009-06-25T15:57:18-05:00 2008-12-05T08:29:45-06:00 Use of the Leica Ultramichrotomes for sectioning. 2 20 1 Ultramicrotome 158 105 79 20 1 51 13 2008-12-05T08:29:45-06:00 2009-10-30T14:27:54-05:00 - 6 112 2 Use of Experion system - - - - 19 85 30 2009-10-30T14:27:54-05:00 2009-06-25T15:56:54-05:00 Volocity image analysis software 5 87 1 Volocity 4.7 - 15 - 7.50 1 - 5 2009-06-25T15:56:54-05:00 2009-11-18T14:43:21-06:00 Fees are waived through December 2009. 11 114 6 Xenogen IVIS Spectrum Imaging - - - - 20 - - 2009-11-18T14:43:21-06:00