Methods of Cell Preparation

There are many good methods described on the Internet. The examples described below will help the inexperienced investigator get started with his or her flow cytometry experiment. It may be necessary to modify these protocols according to individual goals and the type of cells to be analyzed. In addition, the Table of Fluorochromes from the Salk Institute in La Jolla, CA is an excellent resource.

• Labeling Proteins with FITC or PE
• General Immunophenotyping
• Leukocyte Surface Antigens in Whole Blood
• Fixative Preparation
• DNA Analysis of paraffin-embedded tissue
• GFP with fixative
• Apoptosis:
  • Measuring Apoptosis and Necrosis
  • Annexin-V & Propidium Iodide
  • MC540
• Intracellular Ca++ assay
  • Intracellular Calcium
  • Excitation Status