Model No.: LSM 510 Manufacturer: Carl Zeiss, Inc. Location: RRC-West, MSB E-32D

The LSM 510 was installed in March 1998 and has the following specifications:

• Sources of lasers:
  • Argon laser - 458/488, 25mW
  • HeNe laser - 633nm, 5mW
  • UV laser - 351/364nm, 80mW
• Axiovert 1000M microscope - fully motorized and integrated microscope control. Objectives:
  • Achrostigmat 10x/0.25 PH1 (not for laser scanning).
  • Plan-neo 25x/0.8 IMM. corr.
  • C-apochromat 40X/1.2 W.corr.
  • C-apochromat 63x/1.2 W.corr.
  • Plan-neo 100x/1.3 OIL WD-0.06.
• Four confocal channels for reflected light (blue, green, red and dark red), plus one for transmitted light (for DIC). All 4 channels can be recorded and displayed simultaneously or separately.
• Multitracking scanning can efficiently prevent the cross talk between different channels. Users can multiplex up to 4 configurations and collect images in up to 8 output channels.
• Image processing functions include Z sections, standard 2D/3D, 3D reconstruction capabilities and 3D measurement functions.
• "3D for LSM" software upgrades LSM with the functions:
  1. High-performance automated measuring functions for the quantitative analysis of 3D image data.
  2. 3D Reconstruction function allows 3-dimensional views of image data from any required angles.
• Time lapse imaging allows kinetic studies of live cells with green fluorescent protein or calcium indicator studies with Fluo-3 or Ca Green, etc.
• Computer:
  • Intel-based system running Microsoft Windows NT 4.0
  • 128MB RAM
  • 1.44MB 3.5" floppy drive
  • Iomega 100MB ZIP drive
  • Iomega 1GB JAZ drive

Images may also be transferred to a RRC fileserver for temporary storage. This allows users to retrieve (via a FTP program) their data using their office or lab computer.