Model No.: EPICS Elite ESP Manufacturer: Beckman Coulter Location: RRC-West, MSB E-25

The EPICS Elite ESP, purchased new by the Research Resources Center (RRC) and the Office of the Vice Chancellor for Research (OVCR) in 1995, is located in room E-25 of the Medical Sciences Building. In the spring of 1998, a fifth PMT (Photomultiplier Tube) and a red He-Ne laser were added to the instrument; consequently, it is now possible to use fluorochromes that are excited by the red end of the visible spectrum and conduct four color experiments.

Dual Excitation Sources:

1. 15mW air cooled Argon Ion Laser at 488nm: Used to excite Fluoroscein (FITC), Phycoerythrin (PE), PE-Cy5 (Cychrome/Tricolor), PE-Texas Red (ECD/Red 613), Red 670, Rhodamine 123, Propidium Iodide (PI), Acridine Orange, and other compounds.



2. 10mW air cooled Helium-Neon Laser at 633nm (red): Used to excite Allophycocyanin (APC) and Cy5. If APC or Cy5 is used, they should be bright compared to your other reagents in a multicolor experiment.

Helpful suggestions: If cells are to be analyzed on this instrument, it is best that the concentration of cells/particles is 1-5 million cells/ml. Sorting concentrations may be higher especially when the enrichment of rare cells/particles is desired. The cells should be placed in 12 x 75 mm tubes (minimum volume of 250 µl). Sorted cells can be deposited into either 15 ml conical centrifuge tubes or 12 x 75 mm tubes. However, it is easier to sort into the smaller tubes. These tubes should be 75% filled with medium or PBS containing high amounts of protein (i.e. fetal calf serum, bovine serum albumin, etc.).

For more details on what you need for sorting refer to our Sort Protocol page. A sort worksheet (PDF format) or an analysis worksheet (PDF format) must be filled out for every sort or analysis performed by RRC-FCS personnel. If you acquire/analyze the data yourself, this step may not be necessary.