Model No.: LTQ FT Manufacturer: Thermo Finnigan Location: RRC-West, 552 PHARM

The Thermo Finnigan LTQ FT hybrid linear ion trap - Fourier Transform ICR mass spectrometer provides high resolution, accurate mass determinations, and MS-n. The FT Ion Cyclotron Resonance analyzer has an actively shielded 7 Tesla superconducting magnet. Ions trapped in the ICR cell are excited by applying a SWIFT (Stored Waveform Inverse Fourier Transform) waveform. Mass range is 50 to 2000 in FT mode. The FT resolution is user selectable from 12,500 (FWHM) up to a maximium mass resolution of 500,000 at m/z 400. It is capable of simutaneous high resolution, mass accuracy, and sensitivity over one mass decade (e.g. m/z 200 - m/z 2000). It has a fast data acquisition rate (1 second repetition rate) with very high resolution (100,000 at m/z 400). The mass accuracy is better than 2 ppm with external calibration. Sensitivity is sub fmol on-column in LC/MS. It is cabable of simultaeous full scan FT MS, data dependent zoom scan, and MS/MS scans. It is equipped with several nanoESI sources (including static, dynamic, and packed tip) with a Pulnix video camera and has the standard Finnigan IonMax API source with the ESI attachment. The ICR cell has an Electron Capture Dissociation (ECD) emitter cathode attached to the back of the cell. The ECD method of fragmentation produces c and z ions. This method preserves co- and post-translational modifications (PTMs) such as phosporalation, O- and N-linked glycosalation and sulfation. The LTQ also has Wide Band Activation which allows the simultaneous fragmentation of a parent ion and its corresponding water loss peak by resonance excitation.

The LC system consists of a Michrom BioResources, Inc. Paradigm AS1 autosampler, MS4 multi-Dimensional Separations module, MG4 Helium sparge unit and variable wavelength single channel UV-Vis detector. The MS4 separations module is equipped with four 5000 psi PEEK pumps (1-5000 µL/min Isocratic flows). It has two 10-port PEEK micro valves, two PEEK micro mixers (18 µL volume with <50 µL sweep out volume), and electronic flow splitter (50 nL/min - 5 mL/min gradients).

The Thermo Finnigan LTQ FT was purchased in 2005 with a grant from the Searle Funds at The Chicago Community Trust for the Chicago Biomedical Consortium for proteomics applications.