The CyAn was obtained by agreement with DakoCytomation when the DakoCytomation MoFlo was purchased. This instrument was installed July, 2005 in room E-25 of the Medical Sciences Building. It is run with DakoCytomation Summit program (v4.3) and equipped with recently improved optics. It has 3 solid state lasers which will enable the user to analyze up to nine colors of fluorescence. This bench top analyzer can acquire/analyze up to 50,000 events/sec. It has multiple sets of filters so that most fluorochromes that are on the market now can be used for experiments. The only fluorochromes that can not be used with this instrument are those that require a UV or 568nm laser line for excitation.

Excitation Sources:

1. A 488 nm solid state blue-green diode laser: Used to excite Fluoroscein (FITC), Phycoerythrin (PE), PE-Cy5 (Cychrome/Tricolor), PE-Texas Red (ECD/Red 613), Red 670, Rhodamine 123, Propidium Iodide (PI), Acridine Orange, and other compounds.



2. A 635 nm solid-state red diode laser: Used to excite Allophycocyanin (APC), Cy5 and the tandem conjugate APC-Cy7.



3. A 405 nm solid-state violet diode laser: This laser is excellent for exciting Pacific Blue, Pacific Orange, Alexa 430, Alexa Fluor 405, Cascade Yellow and the fluorescent protein eCFP.

Helpful suggestions: If cells are to be analyzed on this instrument, it is best that the concentration of cells/particles be around 1-20 million cells/ml.

An analysis worksheet (PDF format) must be filled out for every analysis performed by RRC-FCS personnel. If you acquire/analyze the data yourself, this step may not be necessary.