2009-08-20T10:14:30-05:00

360 MHz 54 mm bore primarily used for routine 1D and 2D experiments w/wo Z gradient. Probe tuning is not needed. Scheduling is arranged via RRC web accessed software (OLISS). 68

AVANCE

360 MHz NMR

116

image/jpeg

NMR-360.jpg

725220

5 mm Quadra Nuclear Probe (QNP) for observing H1, C13, F19 and P31 nuclei. Temperature control precise to 1 degree C is available. Data can be up loaded for off line analysis and archiving.

2009-11-19T09:45:40-06:00

2009-08-20T10:10:28-05:00

500 MHz 54 mm bore operating as a microMRI or multinuclear NMR spectrometer. MRI can analyze samples up to 10mm in diameter or smaller than 1mm. The NMR uses direct or indirect detection w/wo a Z gradient. Temperature control is available precise to 0.1 degree C. All data can be up loaded for off line analysis and archiving. Scheduling is arranged via RRC web accessed software (OLISS). 67

AVANCE

500 MHz NMR/micro-MRI

150

image/jpeg

NMR-500.jpg

91555

MRI uses Paravision 4.0 NMR uses Topspin 1.3. NMR has 3 probes: 5mm Broadband Observe, 5mm Inverse with C13 and N15 decoupler coils and 2.5mm Inverse with C13 and P31 decoupler colis.

2009-11-19T09:41:50-06:00

2009-07-13T10:23:49-05:00

The HB601UX is a 100kV cold field emission dedicated scanning transmission electron microscope (dSTEM). As built this ultra-high vacuum (UHV) instrument is capable of producing a minimum probe of 0.22nm, and has exceptional probe stability and a low energy spread (<0.3eV). The instrument has been upgraded with a Nion Cs aberration corrector and a Gatan Enfina detection system for EELS which should allow sub 0.1nm spatial resolution with 0.3eV energy resolution. At present, with the assistance of the Nanoscale Physics group, we are reinstalling the instrument. Please check back to see when it as available for use 65

HB601UX

Aberration Corrected STEM

image/jpeg

IMG_2839.JPG

1944695

The aim is to achieve a spatial resolution of less than 0.1nm whilst retaining an energy resolution of 0.3eV.

2009-07-14T15:01:01-05:00

2009-10-30T12:39:03-05:00

GeneChip Scanner 3000 7G GeneChip Fluidics Station 450 GeneChip Hybridization Oven 640 69

Affymetrix GeneChip System

2009-10-30T12:39:03-05:00

2008-12-05T13:31:35-06:00

The Analytical Electron Microscope, the JEM-2010F, is a 200kV field emission transmission electron microscope with a Schottky field emission electron source. It is the first of its kind specified to operate primarily in the STEM mode when installed in 1998. It is fitted with an ultra-high resolution pole piece, which in STEM mode is capable of producing a probe size of 0.13nm with 15pA of current. Magnifications in excess of 10,000,000x are possible in this mode. An annular dark field detector allows Z contrast images in STEM with an imaging resolution equal to the probe size. In conventional TEM mode the lattice resolution is 0.1nm , with a magnification range from 50x to 1,500,000x . Analytical capabilities include a Thermo Noran Vantage XEDS system with a 40mm2 light element detector, drift compensation and spectrum imaging, and a Gatan imaging filter (GIF) which allows EELS and energy filtered imaging. Heating (1000 deg C) and cooling (-170 deg C) stages are available for this instrument and the JEM-3010. 8

JEM-2010F

Analytical Electron Microscope (TEM/STEM)

236

159

119

image/jpeg

JEM2010F_2008.jpg

195638

<UL> <li>Resolution: TEM: 0.1nm lattice, 0.19nm point-to-point. STEM: 0.13nm</li> <li>Accelerating Voltage: 80, 100, 120, 160, 200kV. </li> <li>Objective lens: Cs 0.5mm, Cc 1.1mm.</li> <li>Spot sizes: TEM mode 2~5nm, EDS/NBD/CBD: 0.5~2.4nm, STEM mode 0.13~1nm.</li> <li>Magnification range: TEM 2,0000x - 1,500,000x</li> <li>Magnification Range: STEM 10,000x - 8,000,000x</li> <li>Camera length: 80~2,000mm.</li> <li>Specimen Tilt: +/-25 degrees (X, Y)</li> </UL>

2009-06-03T15:41:48-05:00

2009-04-14T12:12:22-05:00

The autoMACS™ Separator is a benchtop instrument for high-speed automated cell sorting. Employing the MACS® Technology, the autoMACS Separator is designed for positive selection as well as depletion of magnetically labeled cells. The autoMACS Separator is operated with pre-set separation programs, thus, allowing optimization of cell sorting approaches according to cell abundance and the intensity of marker expression. The separated cells are immediately ready for experiments, cell analysis, or further subset sorting. 39

AutoMACS

image/jpeg

automacs.jpeg

20596

Fast—up to 4×109 magnetically labeled cells are sorted automatically within a few minutes. • Efficient—rare cells can be enriched up to 10,000-fold or unwanted cells may be depleted up to 99%. • Reliable—proven automated cell separation technology since 1999.

2009-04-14T12:12:22-05:00

2009-07-01T16:23:45-05:00

AIS2 auto-injection module. An additional $35 set-up fee applies. Fee includes use of the LSM 510 META The investigator can operate the system entirely via the computer workstation and inject cells by pointing and clicking which results in high injection rates of about 1,500 cells per hour. The injected cells can be marked for relocating and avoiding double injection. A unique aspect of our AIS2 system is its combination with the LSM 510 META confocal microscope, which allows the user to trace interesting targets with the time-lapse program. These two systems can be rapidly switched between by clicking one button. The combination of confocal microscopy and microinjection techniques bridges the gap between in vivo physiology and in vitro biochemistry and molecular biology. Investigators can study complex cellular processes, structure and function in vivo using single cells. 63

CoppiC INJECT AIS2

Automatic injection system

264

156

132

image/jpeg

AIS2 injector.jpg

10399

2009-07-02T10:05:34-05:00

2009-06-03T17:06:53-05:00

Measures protein association, dissociation rates, equilibrium binding constants 49

T100

Biacore Surface Plasmon Resonance

200

150

100

2009-10-19T17:35:01-05:00

2009-02-16T10:20:19-06:00

The Bio-Rad Bio-Plex flow cytometer, is specially designed to analyze microsphere based multiplex protein assays. This is an alternative to using ELISA assays to determine the concentration of proteins in solution, such as cell lysates. The advantage of doing multiplex assays is that one can get results simultaneously for many proteins dissolved in a very small volume. Using the user friendly Bio-Plex software, standard curves of known concentration of all analytes are automatically computed and all data from the standards and unknowns can be displayed in graphic or table form. The results can also be saved in either form whichever is preferred by the investigator. Kits for simultaneous quantitative measurement of up to 25-30 proteins are available. Examples of proteins that can be analyzed with commercially prepared Luminex microspheres are human/murine/rat cytokines, human/murine phosphoproteins, growth factors, kinases, and transcription factors. Users may also create custom assays for any proteins having corresponding antibodies. 34

Bio-Plex

please call

155

please call

image/jpeg

bio-plex_200_system.small.jpg

25819

Dual Excitation Sources: 1. A 10mW 532nm green diode used to excite the Phycoerythrin (PE)-strepavidin reporter molecule which is the indicator of protein binding to the individual beads. 2. A 10mW 635nm red diode use to excite the beads which emit 2 different wavelengths in varying proportions depending on the spectral address of the individual bead. Helpful hints: It takes several hours to set up the plates for the Bio-Plex, so it is helpful to start early in the morning. You may prepare your plates in room E-25 MSB if you do not have a vacuum manifold in your laboratory.

please call

2009-04-14T11:53:55-05:00

2009-10-30T13:56:36-05:00

- 77

BioRobot 8000

2009-10-30T13:56:36-05:00

2008-07-14T14:33:33-05:00

The LSM 510 was installed in March 1998. An advanced function of the Zeiss LSM 510 microscope is the multi-tracking scanning capability that minimizes cross-talk of fluorescent signals while working with multiple fluorescent targets. It is equipped with four PMT making it possible to completely separate four colors. Images may be transferred to a RRC fileserver for temporary storage. This allows users to retrieve (via a FTP program) their data using their office or lab computer. Free use of offline LSM workstation for image manipulation and analysis after capturing the images 3

LSM 510

Confocal Microscope

210

105

109

image/jpeg

lsm_510.tn.jpg

7710

Sources of lasers <ul> <li>Omnichrome KrAr laser - 458/568, 25mW</li> <li>HeNe laser - 633nm, 5mW</li> <li>Coherent Enterprise UV UV laser - 351/364nm, 80mW</li> </ul> PMT1 emission filters <li>LP 385 (DAPI)</li> <li>LP 505 (GFP, FITC, Alexa 488)</li> <li>BP 505-550 (GFP, FITC, Alexa 488)</li> <li>LP 590 (CY3/Rhod/TxRd)</li> <li>BP 585-615 (CY3/Rhod/TxRd)</li> <li>LP 650 (CY5)</li> <li>BP 470-500 (INDO)</li> PMT2 emission filters <li>BP 385-470 (DAPI)</li> <li>BP 350-430 (INDO)</li> <li> LP LP505 (GFP, FITC, Alexa 488)</li> <li> BP 500-530 (GFP, FITC, Alexa 488)</li> <li> BP 500-550 (GFP, FITC, Alexa 488)</li> <li> BP 585-615 (CY3/Rhod/TxRd)</li> PMT3 emission filters <li>LP 505 (GFP, FITC, Alexa 488)</li> <li>BP505-530 (GFP, FITC, Alexa 488)</li> <li>BP 505-550 (GFP, FITC, Alexa 488)</li> <li>BPP 585-615 (CY3/Rhod/TxRd)</li> PMT4 emission filters <li>BPP 585-615 (CY3/Rhod/TxRd)</li> Axiovert 1000M microscope Fully motorized and integrated microscope control. Objectives: <ul> <li>Achrostigmat 10x/0.25 PH1 (not for laser scanning).</li> <li>Plan-neo 25x/0.8 IMM. corr.</li> <li>C-apochromat 40X/1.2 W.corr.</li> <li>C-apochromat 63x/1.2 W.corr.</li> <li>Plan-neo 100x/1.3 OIL WD-0.06. </li> </ul> Four confocal channels for reflected light (blue, green, red and dark red), plus one for transmitted light (for DIC). All 4 channels can be recorded and displayed simultaneously or separately. Multitracking scanning can efficiently prevent the cross talk between different channels. Users can multiplex up to 4 configurations and collect images in up to 8 output channels. Image processing functions include Z sections, standard 2D/3D, 3D reconstruction capabilities and 3D measurement functions. "3D for LSM" software upgrades LSM with the functions: <ul> <li>High-performance automated measuring functions for the quantitative analysis of 3D image data.</li> <li>3D Reconstruction function allows 3-dimensional views of image data from any required angles. </li> </ul> Time lapse imaging allows kinetic studies of live cells with green fluorescent protein or calcium indicator studies with Fluo-3 or Ca Green, etc. Computer <ul> <li>Intel-based system running Microsoft Windows 2000</li> <li>2.6 G RAM, 1.8 Ghz CPU</li> <li>1.44MB 3.5" floppy drive</li> <li>Iomega 100MB ZIP drive</li> <li>Iomega 1GB JAZ drive </li> </ul>

2009-07-01T09:55:28-05:00

2009-06-24T18:00:28-05:00

Confocal microscope with live cell and AIS2 automated microinjection capabilities The META detection module of the LSM 510 META provides fast acquisition of image stacks with spectral information for every pixel. With its emission fingerprinting technique, it permits the clean separation of several, even spectrally overlapping, fluorescence signals of a specimen such as the separation of GFP and YFP signals. The laser and the configuration settings are suitable for FRET. Free use of Offline LSM workstation for image manipulation and analysis after capturing the images Equipped with Cellbiology Trading CoppiC INJECT automatic injection system (AIS2) Also available: Bioptechs Delta T culture dish system, temperature control live cell system 56

LSM 510 META

Confocal Microscope

210

105

109

image/jpeg

510-meta.200x149.jpg

39841

Seven Lasers lines are available: <li>30 mW Tunable Argon 458/477/488/514nm 30mW (458,477, for CFP; 488 for GFP, CY2, FITC and 514 for green and YFP)</li> <li>1 mW HeNe 543nm (Rhodamine, CY3, Propidium Iodide, Alexa 546)</li> <li>3 mW HeNe 633nm (Dark red such as CY5)</li> <li>25 mW Diode UV 405nm laser for blue dye (BFP, DAPI, Hoechst, AMCA)</li> 2 PMTs (photomultiplier tubes); 1 Meta detector (polychromatic detector, can also be used as another PMT) PMT ChS (Lambda mode) emission filters: 411-750 PMT2 emission filters <li>LP 420 (DAPI)</li> <li>LP 475 (CFP)</li> <li>BP 565-595 (CY3/Rhod/TxRd)</li> <li>BP 420-480 (CFP)</li> <li>BP 470-500</li> <li>BP 475-525</li> <li>vBP 505-530 (GFP, FITC, Alexa 488)</li> PMT3 emission filters <li>LP 505 (GFP, FITC, Alexa 488)</li> <li>LP 560</li> <li>LP 650 (CY5 )</li> <li>BP 625-655 (CY5)</li> <li>BP 505-530 (GFP, FITC, Alexa 488)</li> <li>BP 505-550 (GFP, FITC, Alexa 488)</li> <li>BP 530-560 (YFP)</li> <li>BP 560-615</li> AIS2 auto-injection module: The investigator can operate the system entirely via the computer workstation and inject cells by pointing and clicking which results in high injection rates of about 1,500 cells per hour. The injected cells can be marked for relocating and avoiding double injection. A unique aspect of our AIS2 system is its combination with the LSM 510 META confocal microscope, which allows the user to trace interesting targets with the time-lapse program. These two systems can be rapidly switched between by clicking one button. The combination of confocal microscopy and microinjection techniques bridges the gap between in vivo physiology and in vitro biochemistry and molecular biology. Investigators can study complex cellular processes, structure and function in vivo using single cells. Computer: Intel-based system running Microsoft Windows 2000 Service Pack 2; 2.6 G RAM; 2.4 GHZ CPU

2009-07-01T09:58:30-05:00

2009-07-02T09:18:31-05:00

The Countess™ Automated Cell Counter offers easy and accurate cell counting and viability counts. You can get all the data you need about your cell cultures in just 30 seconds without using a hemocytometer. 64

Cell Counter

Countess Cell Counter

N/A

2.00

n/a

2.00

image/jpeg

countess.jpeg

1925

n/a

2.00

2009-07-02T09:18:31-05:00

2009-01-28T08:59:32-06:00

The CyAn ADP Analyzer is the next step in Advanced Digital Processing with: * Enhanced detection for flow cytometry * State-of-the-art optics and electronics * Automated compensation * Easy to walk-up operation. CyAn ADP 9 Color adds the 405nm solid state laser and two fluorescent channels. Additional research applications include stem cell/SP analysis, DAPI Cell Cycle analysis and CFP-YFP FRET analysis. 30

Cyan ADP

CYAN

170

102

image/gif

CYAN.gif

20468

Laser Light Source Parameters Fluorochromes FITC, GFP, CFSE, Alexa Fluor✝ 488, YFP* 488 nm FL1 FL2 PE ECD, PE-Alexa Fluor 610, PI, Qdot605* FL3 FL4 PC5, PerCP, PC5.5, PerCP-Cy5.5*, 7-AAD, Qdot655* FL5 PC7, PE-Alexa Fluor 750, Qdot705* Pacific Blue✝, Cascade Blue✝, DAPI, CFP* 405 nm FL6 Cascade Yellow✝, Pacific Orange✝, AmCyan*, Qdot565* FL7 642 nm FL8 APC FL9 APC-Cy7, APC-Alexa Fluor 700*, APC-Cy5.5*, Alexa Fluor 700*, Qdot800

2009-04-14T11:51:38-05:00

2009-06-25T15:11:58-05:00

- 62

Cytospin 3

Cytospin

image/jpeg

cytospin.jpg

10128

2009-07-02T10:02:23-05:00

2009-06-25T14:37:54-05:00

Deltavision Deconvolution Microscope. This microscope excels with certain types of imaging, especially Z-series, 3D/rotatable reconstructions, time course series, and multiple-color fluorescence microscopy. The Microscope is currently managed by Nancey Freitag, Department of Microbiology and Immunolgy. For access to the microscope, please contact her at: 312-355-4903 nfreitag@uic.edu 61

DeltaVision

DeltaVision Deconvolution Microscope

2009-06-26T09:43:19-05:00

2009-06-24T18:17:55-05:00

Digital bright field microscope for general applications. Fee includes use of software: AIM image browser, Potoshop 7.0, Image J and AxioVersion 4.7 57

BX51-TF5

Digital Color Microscope

image/jpeg

Olympus2.jpg

10307

Equipped with DP71 high resolution cooled digital camera

2009-07-02T10:15:14-05:00

2009-06-19T13:44:37-05:00

The EMS also has Gatan hot and cold double tilt stages designed for the JEM-2010F and JEM-3010. The cold stage is liquid nitrogen cooled and can reach a temperature of -170 deg C. Thermal drift after 45 minutes is less than 0.1nm/second. The maximum tilt for this stage is 18 degrees. The heating stage can be used up to 1000 degrees C. Above 500 deg C additional water cooling is necessary. The temperature is reached within 1 minute and after 10 minutes drift rates should be less than 0.2nm/second. The maximum tilt for this stage is 8 degrees in X and 6 degrees in Y. There is no additional charge to use these stages on top of the hourly microscope use charge. Both stages need conditioning the day before use - please e-mail EMS staff in advance if you want to use these stages 50

636 & 652

Double Tilt Cooling and Heating Stages

image/jpeg

Heatingstage.jpg

66102

Cold Stage, double tilt (Model 636)<UL><LI> liquid nitogen cooled minimum temp temperature of -170 deg C.</LI><LI> Thermal drift after 45 minutes - less than 0.1nm/second.</LI><LI> The maximum tilt for this stage is 18 degrees.</LI><UL> Hot Stage, double tilt (Model 652)<UL><LI> max temp 1000 degrees C. (Above 500 deg C additional water cooling necessary).</LI><LI> After 10 minutes at temperature drift rates should be less than 0.2nm/second.</LI><LI> The maximum tilt for this stage is 8 degrees in X and 6 degrees in Y.</LI><UL>

2009-07-13T10:34:54-05:00

2009-02-16T10:30:35-06:00

The EPICS Elite ESP is the latest generation of the Elite product line. This high-performance instrument is a powerful tool for innovative research in immunobiology, cell physiology, molecular biology, genetics, water quality and plant cell physiology. The Elite ESP - Enhanced System Performance - features the capability to analyze up to 4 simultaneous colors of immunofluorescence and perform equally complex multi-parameter applications such as DNA quantitation, physiologic measurements, chromosome enumeration and the study of the hematopoetic process. The cell sorting capability of the system allows for the rapid separation of very large numbers of specific cell populations from a heterogeneous mixture. Extremely high purities -greater than 99 percent - are achieved, even at high rates of analysis. 35

EPICS Elite ESP

ELITE

170

102

image/jpeg

Coulter Epic Elite ESP.jpg

27888

Dual Excitation Sources: 1. 15mW air cooled Argon Ion Laser at 488nm: Used to excite Fluoroscein (FITC), Phycoerythrin (PE), PE-Cy5 (Cychrome/Tricolor), PE-Texas Red (ECD/Red 613), Red 670, Rhodamine 123, Propidium Iodide (PI), Acridine Orange, and other compounds. 2. 10mW air cooled Helium-Neon Laser at 633nm (red): Used to excite Allophycocyanin (APC) and Cy5. If APC or Cy5 is used, they should be bright compared to your other reagents in a multicolor experiment. Helpful suggestions: If cells are to be analyzed on this instrument, it is best that the concentration of cells/particles is 1-5 million cells/ml. Sorting concentrations may be higher especially when the enrichment of rare cells/particles is desired. The cells should be placed in 12 x 75 mm tubes (minimum volume of 250 µl). Sorted cells can be deposited into either 15 ml conical centrifuge tubes or 12 x 75 mm tubes. However, it is easier to sort into the smaller tubes. These tubes should be 75% filled with medium or PBS containing high amounts of protein (i.e. fetal calf serum, bovine serum albumin, etc.).

2009-04-14T12:16:28-05:00

2009-10-30T12:43:57-05:00

Automated Electrophoresis Station Priming Station Vortex Station 70

Experion Automated Electrophoresis System

$30/run

2009-10-30T12:43:57-05:00

2009-01-27T08:26:58-06:00

The BD FACSCaliburTM system is designed specifically to support a wide range of applications, the BD FACSCalibur system is a fully integrated multiparameter system. The BD FACSCalibur system combines unique dual-laser technology and powerful easy to use software. The modularity and innovative technology designed into the BD FACSCalibur system also offers investigators the performance and flexibility required for a variety of research applications. The built-in versatility of the BD FACSCalibur flow cytometer meets the current demands of both clinical and research environments. Its flexibility will help you meet tomorrows demands. 25

FACSCalibur

170

image/jpeg

facscalibur.jpg

8268

Excitation Source: 15mW air cooled Argon Ion Laser at 488nm: Used to excite Fluoroscein (FITC), Phycoerythrin (PE), PE-Cy5 (Cychrome/Tricolor), Red 670, Rhodamine 123, Propidium Iodide (PI), Acridine Orange, and other compounds. You can not acquire more than 3 colors on this instrument. PE-Texas-Red (ECD/Red 610) can not be analyzed. In addition, there is limited compensation (you can not compensate every color on every other color like you can on the BD LSR). Helpful suggestions: If cells are to be analyzed on this instrument, it is best that the concentration of cells/particles be around one/five million cells per milliliter. It is recommended that the cells be put in Falcon 352052 12 x 75 mm round bottom polystyrene tubes with a minimum volume of 500 microliters. Many 12 x 75 mm tubes will not work on this instrument, so if you are not sure of your tubes, test them out before setting up your experiment.

2009-04-14T12:24:07-05:00

2008-12-05T13:50:52-06:00

The Field Emission SEM, the JSM-6320F, is a high resolution SEM with a cold field emission source. High resolutions at low accelerating voltages are possible with this instrument due to its objective lens design. A secondary electron detector is integrated into the bore of the lens and the specimen can be bought up into the lens field. Working distances (WD) of as low as 2mm are possible. The microscope is also fitted with a Noran Voyager EDX system with a light element X-ray detector (15mm WD) and an Autrata Back Scatter Detector. There is also a second conventional secondary electron detector below the lens, which gives more topographic images. It was installed in 1997. Digital images are recorded using a JEOL Orion system reading into a Windows XP computer. 10

JSM-6320F

Field Emission SEM

236

159

119

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JSM-6320F_nu.jpg

163091

<UL> <li>Accelerating Voltage 0.5-30kV</li> <li>Secondary Electron image resolution: 1.2nm (15kV), 2.5nm (1kV).</li> <li>Magnification: 500x ~ 650,000x. </li> <li>Imaging modes: Secondary, Backscattered. </li> <li>Specimen movements: 50mm (X), 70mm (Y), 24mm (Z). </li> <li>Tilt: -5 degrees - 45 degrees.</li> <li>Rotation: 360 degree.</li> <li>Maximum Specimen size: 32mm diameter, 10mm high.</li> </UL>

2009-06-03T15:45:24-05:00

2009-05-29T12:04:59-05:00

A server dedicated to running the Genetics Computer Group (GCG) SeqLab sequencing analysis software package. 45

GCG SeqLab

GCG Server

n/a

Access to the server is done via a SSH client.

n/a

2009-06-06T01:30:09-05:00

2009-01-16T12:54:07-06:00

The Thermo Finnigan LTQ FT hybrid linear ion trap - Fourier Transform ICR mass spectrometer provides high resolution, accurate mass determinations, and MS-n. The FT Ion Cyclotron Resonance analyzer has an actively shielded 7 Tesla superconducting magnet. It is equipped with several nanoESI sources (including static, dynamic, and packed tip) with a Pulnix video camera and has the standard Finnigan IonMax API source with the ESI attachment. The ICR cell has an Electron Capture Dissociation (ECD) emitter cathode attached to the back of the cell. The ECD method of fragmentation produces c and z ions. This method preserves co- and post-translational modifications (PTMs) such as phosporalation, O- and N-linked glycosalation and sulfation. The LTQ also has Wide Band Activation which allows the simultaneous fragmentation of a parent ion and its corresponding water loss peak by resonance excitation. The LC system consists of a Michrom BioResources, Inc. Paradigm AS1 autosampler, MS4 multi-Dimensional Separations module, MG4 Helium sparge unit and variable wavelength single channel UV-Vis detector. The MS4 separations module is equipped with four 5000 psi PEEK pumps (1-5000 µL/min Isocratic flows). It has two 10-port PEEK micro valves, two PEEK micro mixers (18 µL volume with <50 µL sweep out volume), and electronic flow splitter (50 nL/min - 5 mL/min gradients). The Thermo Finnigan LTQ FT was purchased in 2005 with a grant from the Searle Funds at The Chicago Community Trust for the Chicago Biomedical Consortium for proteomics applications. Please visit <a href="http://proteomics.rrc.uic.edu/">the Proteomics & Informatics Services Facility web site</a> for rates and to learn how to schedule the use of the instrument. 21

LTQ FT

ICR LC Mass Spectrometer

inquire

170

130

image/jpeg

LTQ-FT.jpg

50262

Ions trapped in the ICR cell are excited by applying a SWIFT (Stored Waveform Inverse Fourier Transform) waveform. Mass range is 50 to 2000 in FT mode. The FT resolution is user selectable from 12,500 (FWHM) up to a maximium mass resolution of 500,000 at m/z 400. It is capable of simutaneous high resolution, mass accuracy, and sensitivity over one mass decade (e.g. m/z 200 - m/z 2000). It has a fast data acquisition rate (1 second repetition rate) with very high resolution (100,000 at m/z 400). The mass accuracy is better than 2 ppm with external calibration. Sensitivity is sub fmol on-column in LC/MS. It is cabable of simultaeous full scan FT MS, data dependent zoom scan, and MS/MS scans.

2009-10-19T17:31:57-05:00

2009-05-29T11:13:55-05:00

General purpose imaging station equipped with various imaging editing and analysis software. 42

Imaging Station

Windows XP Professional & Linux

2009-05-29T11:57:28-05:00

2009-05-14T14:25:42-05:00

The Xenogen IVIS® Spectrum in vivo imaging system from Caliper Life Sciences uses Xenogen’s novel patented optical imaging technology to facilitate non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals. Quantitative monitoring of both bioluminescent and fluorescent reporters across the blue to almost infrared wavelength spectrum is possible. NOTE: GFP is not recommended for most fluorescent imaging applications; alternatively, plan to use a red shifted fluorescent protein such as cherry, tomato, or katushka. The Spectrum is equipped with a gas anesthesia unit that allows continuous administration of isoflurane gas for anesthetizing animals during imaging. The system’s Living Image 3.1 image acquisition and analysis software includes features for spectral unmixing to remove unwanted autofluorescence and to observe and separate several fluorescent and bioluminescent reporters within the same animal. It can also create 3D tomography images that permit source localization and concentration, as well as anatomical analysis using a 3D mouse atlas. <H3>FEATURES</H3> <UL> <LI>High-sensitivity in vivo imaging of fluorescence and bioluminescence</LI> <LI>High throughput (5 mice) with 23 cm field of view</LI> <LI>High resolution (to 20 microns) with 3.9 cm field of view</LI> <LI>Twenty eight high efficiency filters spanning 430 – 850 nm - Supports spectral unmixing applications - Ideal for distinguishing multiple bioluminescent and fluorescent reporters </LI> <LI>Optical switch in the fluorescence illumination path allows reflection-mode or transmission-mode illumination</LI> <LI>3D diffuse tomographic reconstruction for both fluorescence and bioluminescence that can be analyzed in an anatomical context using The Digital Mouse Atlas.</LI> <LI>Ability to import and automatically co-register CT or MRI images yielding a functional and anatomical context for your scientific data.</LI> <LI>NIST traceable absolute calibrations</LI> <LI>Gas anesthesia inlet and outlet ports</LI> </UL> The instrument is equipped with the XGI-8 Gas Anesthesia Module, which integrates efficient gas delivery while minimizing excess gas exposure to lab personnel into a single system. The Gas Anesthesia System delivers isoflurane gas to a 5-port anesthesia manifold housed in the IVIS System imaging chamber. FOR MORE INFORMATION ON THE SPECTRUM'S FEATURES AND IMAGING APPLICATIONS visit Caliper Life Sciences at <A HREF="http://www.caliperls.com/products/optical-imaging/ivis-spectrum.htm">http://www.caliperls.com/products/optical-imaging/ivis-spectrum.htm</A>. Also visit our wiki page at <A HREF="http://wiki.rrc.uic.edu/wiki/RRC-TPS:_User_Policies">http://wiki.rrc.uic.edu/wiki/RRC-TPS:_User_Policies</A>. 40

IVIS Spectrum

IVIS Imaging System

0.00

image/jpeg

IVISspectrumCrop.jpg

8908

Power Requirements 20 Amps for 120 VAC or 10 Amps for 230 VAC Dimensions •Imaging Chamber Interior Size: 43 x 50 x 60 cm (W x D x H) •Imaging System Space Requirement: 203 x 163 x 214 cm (W x D x H) Camera Sensor Back-thinned, back-illuminated Grade 1 CCD CCD Size 2.7 x 2.7 cm Operating Temperature -90°C Imaging Pixels 2048 x 2048 Quantum Efficiency >85% 500 – 700 nm; >30% 400 – 900nm Pixel Size 13.5 microns Minimum Detectable Radiance 70 photons/s/sr/cm2 Field of View (FOV) Minimum FOV 3.9 x 3.9 cm Maximum FOV 23 x 23 cm Minimum Image Pixel Resolution 20 microns Read Noise < 3 electrons for bin=1,2,4; < 5 electrons for bin=8,16 Dark Current (Typical) <100 electrons/s/cm2 Filter Slots Fluorescence Excitation 12 Fluorescence Emission 24 Fluorescence Filters Excitation 10 Emission 18 Heated Chamber Yes Diffuse Tomography Software Yes Gas Anesthesia Yes Computer 2.8 GHz, 1 GB RAM, RW CD/DVD, 80 GB HD, 20” flat screen Lens f/1 – f/8, 1.5x, 2.5x, 5x, 8.7x magnifications Stage Temperature 20 – 40°C Transillumination Stage Yes Workbench Yes

0.00

2009-10-02T02:30:20-05:00

2009-06-24T12:33:32-05:00

The total internal reflection fluorescence microscope (TIRFM) enables very high-resolution imaging of signals near biological membranes. Thin layer specimens, usually less than 200 nm, can be observed. Includes incubator for live-cell imaging over extended times. Users from the Department of Pharmacology contact Oleg Chaga, PhD: 996-6057; Ochaga@uic.edu Policies: *For use as standard fluorescence scope a reduce rate of $15/hour applies. All prospective users are expected to have experience with basic epi-fluorescence microscopy. Everyone must be trained and approved for independent operation of the system by Mei Ling Chen (users from UIC and outside UIC) or Oleg Chaga (users from the Department of Pharmacology). The average training time is approximately 4 hrs, depending on the user’s experience with digital microscopes. Everyone is expected to use and care for the equipment as demonstrated during the initial training session. Abuse of the system may result in the cancellation of user privileges; repair costs not covered by the service contract will be charged to the P.I. Data can be saved to CDs, DVDs, and USB drives. Sign up by calling or email. Users from UIC should wait for email response from Mei Ling Chen. Each appointment is restricted to five days (long use must include 2 days on weekend) per week. Sign up should include user and PI names, RRC account number, time, email address and phone number. Web sign up will be initiated soon. 55

TIRF

Laser-TIRF Imaging System

150

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70780

The Zeiss Axiovert 200M is a modern, motorized, general-purpose inverted microscope suitable for most imaging applications. The system includes a HAMAMATSU C4742-98-24ERG CCD camera and Axiovision 4.7 software. It includes the Total Internal Reflection Fluorescence (TIRF) hardware package from Zeiss, including a multi-line 100mW multi-line (458nm, 488nm, 514nm) Lasos 77 laser. It also is equipped with temperature and CO2 controlled incubator for live-cell imaging over extended times. This is a relatively new imaging modality that is likely to become widely used in cell biology research applications.

15/25*

2009-06-30T09:37:25-05:00

2009-01-16T14:05:11-06:00

The Shimadzu LCMS-IT-TOF is a hybrid mass spectrometer coupling atmospheric pressure ionization with Ion-Trap (IT) and Time-of-Flight (TOF) technologies. It delivers high mass accuracy and high mass resolution independent of MS mode. 23

LCMS IT-TOF

LCMS IT-TOF Mass Spectrometer

inquire

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66440

Resolution is 10,000 at m/z 1000 independent of MS mode. The LCMS-IT-TOF has been designed to maximize sensitivity and selectivity by optimizing the ion transport to the TOF analyzer and redefining the capability of the quadrupole ion trap. The ion trap is used to focus ions before ejection into the TOF as well as supporting MSn analysis with effective precursor ion selection capabilities (resolution > 1,000 at 1,000 m/z). Compressed Ion Injection" (CII) (patented) The CII method makes it possible to control the accumulation of ions before they are introduced into the ion trap. Adopted for the LCMS-IT-TOF, it effectively couples the LC system to the MS, dramatically increasing sensitivity. Ballistic Ion Extraction (BIE) (patented) The use of BIE accelerates ions into the TOF instantly, and combined with the Dual-Stage Reflectron (DSR) (patented), achieves faster, more stable, and higher resolution spectra. Temperature Control of the Flight Tube In concert with the localized ion due to BIE from the QIT and the 10bit high-speed transient recorder, the temperature control mechanism of the flight tube enables extended stability in mass accuracy. High-speed Ion Polarity Switching Utilizing a newly developed, accurate and stable power supply, in combination with an innovative high-voltage switch, allows for polarity switching in only 0.1 sec or less.

2009-10-19T17:30:12-05:00

2009-06-19T14:51:40-05:00

There are many ways of preparing specimens for electron microscopy. The EMS staff are available to help investigators determine the best way of getting the information they need from their specimens. The following is intended only as a general guideline. The size of the SEM specimen depends on which instrument you need for your analysis. The Hitachi S-3000N is able to accept specimens up to 150mm in diameter (although parts of the specimen cannot be accessed). The JEOL JSM-6320F can accept specimens up to 30mm in diameter and 10mm high. In general, however, the amount of material should be kept as small as possible, especially if it is non-conducting. Conducting specimens can be imaged in both SEMs without any further specimen preparation. Non-conducting specimens may need coating with a conducting film in order to reduce charging at high vacuum in all microscopes. The EMS is able to coat with either carbon, platinum/gold, platinum/palladium or, for high resolution imaging, chromium. In low vacuum mode (S-3000N only) it is not necessary to coat any specimen and some non-conducting specimens can be imaged without charging at low voltage (S-3000N, JSM-6320F). All EMS TEMs take standard size specimens (3mm in diameter). The maximum thickness of the specimen depends on the density of the specimen. The EMS has extensive specimen preparation facilities to convert bulk material into transmission thin samples of the correct diameter. Life Science Specimens are supported on a grid or a slotted 3mm washer. Life Science specimens are typically prepared using an ultra-microtome to cut slices less than 100nm thick which can then be supported on a grid. All life science specimens (for SEM or TEM) need to be processed to remove the water from the specimen and replace it with a plastic resin without introducing artifacts. Typically, for TEM, this is a multistage process involving<UL><LI>Fixation - to halt degradation of tissue</LI><LI>Dehydration - to replace water with ethanol and then a transitional solvent</LI><LI>Infiltration - to impregnate the specimen with resin</LI><LI>Embedding - to enclose the resin impregnated specimen in more resin</LI><LI>Ultramicrotomy - to cut transmission thin slices from the specimen</LI><LI>Staining - to increase the contrast of the images obtained by staining parts of the structure with a heavy metal stain (eg uranyl acetate) 54

EMS-W

Life Science and SEM Specimen Preparation

158

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Equipment available in the EMS-W prep lab includes:-<UL><LI>Sputter coaters for depositing metal films (Cressington 208HR (Cr) and Polaron E5100 (Pt/Pd & Au/Pt)).</LI><LI>Carbon Coater (Edwards E306A) </LI><LI>Tissue Rotator (EMS), Centrifuge (Spectrafuge 6C)</LI><LI>various Ovens</LI><LI>Glass Knife Maker (LKB 7801B) </LI><LI>Ultramicrotomes (Leica Ultracut UCT and Reichart Ultracut E) </LI><LI>Leica Automatic Freeze Substitution (AFS) </LI><LI>Reichart Metal Mirror Cryofixation System (MM80E) and Universal Cryofixation System (KF80) </LI><UL>

2009-06-19T15:34:59-05:00

2008-12-05T12:58:25-06:00

The Life Science TEM, the JEM-1220, is a 120kV transmission electron microscope fitted with a tungsten electron source which was installed in 1997. For Life Science applications it is normally operated at 80kV. The instrument has a Gatan Es1000W 11MP CCD camera above the viewing chamber, which reads out into a Windows XP computer using Digital Micrograph software. 6

JEM-1220

Life Science TEM

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<UL> <li>Resolution: 0.2nm lattice; 0.36nm point to point.</li> <li>Accelerating Voltage: 40, 60, 80, 100, 120kV.</li> <li>Goniometer Tilt Range: +/-30 degrees.</li> <li>Magnification range: 50x-600,000x.</li> </UL>

2009-06-03T15:44:47-05:00

2009-02-16T10:11:37-06:00

Building on the ease of use and flexibility, the BD™ LSR is the first UV benchtop analyzer. It is equipped with BD CellQuest pro software which is familiar to many investigators who use the instrument unassisted. This instrument is capable of analyzing a six color experiment. Four of the colors are acquired with 488 nm Argon excitation, with the remaining two colors acquired with Helium-Cadmium excitation. 33

LSR I

LSR

170

102

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Dual Excitation Sources: 15mW air cooled Argon Ion Laser tuned to 488nm: Used to excite Fluoroscein (FITC), Phycoerythrin (PE), PE-Cy5 (Cychrome/Tricolor), PE-Texas Red (ECD/Red 613), Red 670, Rhodamine 123, Propidium Iodide (PI), Acridine Orange, and other compounds. 8mW air cooled Helium-Cadmium laser emitting light at 325nm: Especially useful to measure calcium flux with Indo-1 AM ester. This laser can also be used to excite DNA specific dyes such as Hoechst and DAPI.

2009-04-14T12:14:16-05:00

2009-05-19T14:56:03-05:00

The LTQ is a state of the art linear ion trap mass spectrometer equipped with a two dimensional HPLC system. This instrument can be used for tandem mass spectrometry based protein identification or quantitation. It is located in Pharmacy Room 552. The LTQ is a high performance linear ion trap based instrument equipped with a Dionex Ultimate 3000 Nano-HPLC, Nano and conventional electrospray spray chambers, and an APCI spray chamber. Features: <ul> <li>Ultra fast Cycle Time <li>MSn capability <li>Automated Data Dependent Neutral Loss for PTM determination </ul> Applications: <ul> <li> BioMarker Studies <li> Protein identification using conventional peptide microsequencing protocols <li> Protein Expression/Quantitation studies <li> Post Translational Modification detection </ul> Please visit <a href="http://proteomics.rrc.uic.edu/">the Proteomics & Informatics Services Facility web site</a> for more details and to learn how to schedule use of the instrument. 41

LTQ

LTQ LC/MS/MS

inquire

150

110

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45932

It is equipped with several nanoESI sources (including static, dynamic, and packed tip) with a Pulnix video camera and has the standard Finnigan IonMax API source with the ESI attachment

2009-10-19T17:31:21-05:00

2009-01-16T12:17:48-06:00

The JEOL GCMate II is a high resolution double focusing magnetic sector benchtop GC mass spectrometer equipped with ion sources for electron impact (EI), chemical ionization (CI) and fast atom bombardment (FAB) by direct insertion probe and is capable of exact mass measurements for elemental composition determinations. The spectrometer is equipped with high energy Collision Induced Dissociation (CID) linked scan unit allowing MS-MS fragmentation experiments (product ion scans, precursor scans, constant neutral loss scans) for use in structural analysis. The mass spectrometer's data processing software automatically bridges to the NIST Mass Spectral Database Library for searching unknown compounds. An Agilent 6890 GC with Electronic Pressure Control and a split/splitless capillary column injector interfaced to the GCMate allows GC/MS by EI or CI for mixture analysis. An Agilent 7673 Automatic Liquid Sampler (ALS) with 100 vial tray attached to the GC allows unattended sample injections. The JEOL GCMate II was installed in 2002 and was purchased from internal funds. 18

GCMate II

Magnetic Sector GC Mass Spectrometer

162

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Mass range is from a low of m/z 1 up to m/z 1000 at full accelerating voltage and up to m/z 3000 at reduced accelerating voltage. Resolution is user selectable from 500 up to 5000. Sensitivity is 30 pg of methyl stearate injected into the GC column producing a S/N of 10:1 at a resolution of 500 for the molecular ion, m/z 298.

2009-06-23T10:24:35-05:00

2009-01-16T11:02:03-06:00

The Applied Biosystems, Inc. Voyager-DE PRO high performance benchtop matrix-assisted laser desorption ionization/time-of-flight (MALDI/TOF) mass spectrometer provides high mass range, high sensitivity, and high throughput MALDI/TOF mass spectra of biopolymers such as proteins and oligonucleotides as well as organic prepolymers and other large organic molecules. The Applied Biosystems, Inc. Voyager-DE PRO PS1 was purchased in 2002 with an NIH grant to Dr. Richard B. van Breemen. Billing is done through the Proteomics & Informatics Services Facility - please visit <a href="http://proteomics.rrc.uic.edu/index.php?option=com_content&view=article&id=53&Itemid=66">the Service Price List on their web site</a> for more details and to schedule the instrument. 15

Voyager-DE Pro

MALDI-TOF Mass Spectrometer

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35349

Mass range is over 300,000 with resolution in linear mode > 1000 for myoglobin and resolution in reflectron mode > 7000 for angiotension. It uses a 337 nm nitrogen laser. Sensitivity is 5 fmol of neurotensin. It is suitable for proteomics applications such as peptide mapping, peptide sequencing using post-source decay (PSD), and protein identification. The Proteomics Solution 1 (PS1) System consists of a stand-alone computer workstation for database searching as part of proteomics protein identification and a robotic workstation that can prepare samples from 96-well plates for high throughput by spotting a 100 well plate with sample, reference, and MALDI matrix.

2009-06-24T09:46:51-05:00

2009-02-12T12:33:19-06:00

The Applied Biosystems 4700 Proteomics Analyzer with TOF/TOF™ Optics acquires MS and MS/MS spectra in a high-throughput, easy-to-use MALDI platform. It supports a wide variety of front-end sample preparation methods, such as 1-D and 2-D gels, multi-dimensional liquid chromatography (MDLC), or Applied Biosystems proprietary Isotope Coded Affinity Tag technology, as well as back-end bioinformatics platforms. The 4700 Proteomics Analyzer has a coaxial tandem time-of-flight MS/MS configuration providing high-energy collision-induced dissociation for fragmentation with structural information such as immonium ions, side-chain cleavages, and internal fragments. This structural detail can elucidate posttranslational modifications and leucine/isoleucine differentiation and other complexities within high-resolution proteomics. The large mass range and high-energy fragmentation of the TOF/TOF™ optics can analyze proteins without prior digestion. The high rep-rate laser and high-speed data acquisition system provide high sensitivity and dynamic range. 32

4700 Proteomics Analyzer

MALDI-TOF/TOF Mass Spectrometer

inquire

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ABI 4700.jpg

33531

The Applied Biosystems 4700 Proteomics Analyzer also includes a 200 Hz laser and acquisition system.

2009-10-19T17:30:49-05:00

2009-06-19T14:32:15-05:00

EMS-E

Materials Science Specimen Preparation

158

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181015

Equipment available in the EMS-E prep lab includes:- <UL><LI>Diamond saw for slicing (South Bay 650) </LI><LI>Ultrasonic Cutter, Disc Cutter and Rotary Slurry Cutter for preparing 3mm discs (South Bay 380, 310 and 360) </LI><LI>Polishing Wheels (South Bay 900 and 910T) </LI><LI>Tripod Polishers (South Bay 590W) and Disc grinders (Gatan 623) </LI><LI>Dimpler/ Polisher (South Bay 515) </LI><LI>Plasma Cleaner/ Etcher (South Bay PC150) </LI><LI>Twin Jet Electropolisher (Fischione 110) </LI><LI>Ion Mills (Fischione 1010 and 3000, Gatan Duo Mill) </LI><LI>Ultramicrotome (Leica UCT) </LI><LI>Chemical Thinning Apparatus </LI><LI>Vacuum Evaporator, Carbon & Metal (JEOL JEE-4X)</LI><UL>

2009-06-19T15:33:28-05:00

2008-12-05T13:13:03-06:00

The Materials Science TEM, the JEM-3010, is a 300kV transmission electron microscope with a LaB6 electron source which was installed in 1998. It is fitted with an ultra-high resolution pole piece. Images are collected using a Gatan digital imaging system with a Windows XP computer running Digital Micrograph software. There are two cameras, an intensified TV camera for set up and a multi-scan 1Kx1K CCD camera. The microscope is also fitted with a Thermo Noran Vantage XEDS system with a light element X-ray detector. Heating (1000 deg C) and cooling (-170 deg C) stages are available for this instrument and the JEM-2010F. 7

JEM-3010

Materials Science TEM

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<UL> <li>Resolution: 0.24nm lattice, 0.17nm point-to-point.</li> <li>Accelerating Voltage: 100, 150, 200, 250, 300kV.</li> <li>Objective lens: focal length 2.5mm, Cs 0.6mm, Cc 1.3mm, minimum focus step 1nm.</li> <li>Spot Size: TEM mode 200~20nm dia (5 steps), EDS/NBD/CBD mode 25~1.0nm (8 steps).</li> <li>Magnification Range: 4,000x - 1,500,000x.</li> <li>Camera length range: 120 - 3,000mm.</li> <li>Specimen Tilt range +/-20 degrees (X & Y).</li> <li>Specimen movement: 2mm (X,Y), 0.2mm (Z)</li> </UL>

2009-06-03T15:44:21-05:00

2009-02-16T12:29:14-06:00

The MoFlo has set the standard for cell sorting with both functionality and speed. It is designed for researchers with features of improved productivity, an array of bio-safety features and easy-to-use software. This high-speed, configurable system has solid state lasers, high viability, high yield, an analysis rate of 100,000 events per second and sort rates of up to 70,000 events per second. Experience extremes while maintaining high performance, flexibility and modularity. 36

MoFlo

232

116

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2731

Excitation Sources : 1. 5W water cooled Argon Ion Laser tuned to 488 nm: This common laser is used to excite Fluoroscein (FITC), Phycoerythrin (PE), PE-Cy5 (Cychrome/Tricolor), PE-Texas Red (ECD/Red 613), Red 670, Rhodamine 123, Propidium Iodide (PI), Acridine Orange, and other compounds. In addition, it can be tuned to other commonly used wavelengths such as 514 nm (TRIC) or 457 nm (NBD). Please inform RRC personnel if you need a non-488 nm wavelength to excite your fluorochrome. 2. 5W water cooled Argon Ion Laser tuned to MLUV. This laser is very good for exciting DNA specific dyes such as Hoechst and DAPI, and blue fluorescent protein e-BFP. Calcium flux can be measured using this laser. However, the UV wavelengths from the argon laser are a little too low to effectively excite Pacific Blue,Cascade Blue or eCFP. 3. 4W water cooled Krypton Ion Laser tuned for either violet wavelengths 406/413/415 nm or the yellow 568nm line. When tuned to the violet wavelengths, this laser can excite Pacific Blue, Pacific Orange, Cascade Blue, Alexa 430, and fluorescent protein eCFP. When tuned to the yellow 568nm line, this laser can excite TRITC, Alexa 568, DsRed and X-Rhodamine. When making an appointment for the MoFlo, always indicate the fluorochrome you will be using so that the Krypton laser will be equipped with the correct optics. 4. A 25mW 635 nm solid-state red diode laser: Used to excite Allophycocyanin (APC), Cy5 and the tandem conjugate APC-Cy7. This laser was added to the instrument recently; therefore we can now analyze/sort more multicolor combinations than before. Helpful suggestions: To best utilize the high speed sorting ability of this instrument, the cells should be more concentrated than recommended for the Beckman Coulter Elite. We suggest giving us cells that are 10-30 million cells/ml. Sorting concentrations may be higher especially when the enrichment of rare cells/particles is desired. The cells should be placed in tubes in a minimum volume of 500 µl. Sorted cells can be deposited into either 12 x 75 mm or 17 x 100 mm round bottom centrifuge tubes. These tubes should be 75% full of medium or PBS containing high amounts of protein (i.e. fetal calf serum, bovine serum albumin, etc.).

2009-07-02T09:22:06-05:00

2009-10-30T12:51:36-05:00

- 76

Nanodrop 1000 UV/Vis Spectrophotometer

2009-10-30T13:59:52-05:00

2009-01-16T13:13:13-06:00

The Waters SYNAPT™ Mass Spectrometer is a high resolution quadrupole oa-Tof LC mass spectrometer. 22

Waters SYNAPT

oa-Qtof SYNAPT LC Mass Spectrometer

inquire

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45644

TBD

2009-10-19T17:29:48-05:00

2009-10-30T13:59:11-05:00

- 79

OmniGrid Accent Microarrayer Spotter

2009-10-30T13:59:11-05:00

2008-12-05T14:22:50-06:00

The Applied Epi (now Veeco) GEN II MBE is a single-wafer molecular beam epitaxy system which is used to grow high purity metal oxide films. The growth chamber is held at ultra high vacuum and the metal oxide films are grown at a slow deposition rate which allows them to grow epitaxially. The instrument has 6 effusion cells dedicated to Ba, Fe, Cr, Bi, Sr & Ti and also an oxygen source. It was acquired, used, in 2004 and is operated by Prof Sid Ghosh’s group 14

GEN II

Oxide Molecular Beam Epitaxy

620

310

155

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295253

200

100

2009-07-13T12:16:50-05:00

2009-10-30T13:58:14-05:00

- 78

PrimusHT high-throughput PCR Cycler

2009-10-30T13:58:14-05:00

2009-01-16T12:33:38-06:00

The Thermo Finnigan LCQ classic is a quadrupole ion trap LC mass spectrometer equipped with electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) ion sources, interfaced to a Thermo Separation Products liquid chromatography (LC) system. The LC system consists of a SpectraSYSTEMS SCM 1000 vacuum membrane degasser, AS 3000 autosampler, a P4000 gradient elution pump and a UV 2000 dual-wave length detector. The autosampler has a 120 random vial access capacity. The P4000 is a narrow-bore quaternary low-pressure pump with a 0.5-10 ml/min flow rate range. Conventional mobile phases are methanol/acetonitrile/water. Arrangements for the use of other mobile phases must be made prior to sample submissions. Provision of LC columns is the responsibility of the investigator. Samples may be introduced to the MS either by direct infusion from the built-in syringe pump or subsequent to HPLC separation. The operation of both the LC and the LCQ are controlled by a PC based data system. The BioExplore software allows for deconvoluting the spectrum of a multiply-charged protein or peptide m/z charge state envelope into the corresponding uncharged mass spectrum. The LCQ Classic was installed in 1998 and was obtained on an NSF grant by Drs. Richard B. van Breemen, Stuart Scheppele and Charles E. Brown. Acknowledgement Section of Manuscripts If your publication includes data/results/mass spectra obtained with the LCQ mass spectrometer, the following statement, or similar, must be included in the Acknowledgements Section. The LCQ mass spectrometer was purchased in part with a grant from the National Science Foundation (BIR-9111391). 19

LCQ Classic

Quadrupole Ion Trap LC Mass Spectrometer

162

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4501

Positive and negative ion ESI and APCI mass spectra can be acquired over the mass to charge range m/z 50 to m/z 2000 at unit resolution and over a m/z range at 10,000 resolution with a peak width of 0.25 daltons/z which allows identification of ions possessing up to 4 charges. A specific ion may be selected, stored in the trap and collided with He gas in an attempt to have it undergo fragmentation reactions. The spectrum of the fragment ions resulting from such collisions can then be obtained. Also, one of these fragment ions can be selected, stored in the trap and induced to undergo collision-induced fragmentation. This process may be repeated up to 10 times. This MS/MS technique is important in structure determinations because ESI and APCI mass spectra are characterized by the presence of molecular and/or adduct ions (e.g., the molecular ion coordinated with one or more ubiquitous Na ions) and the absence of fragment ions that are often of diagnostic value in structure determination.

2009-06-23T10:25:39-05:00

2009-06-03T12:37:01-05:00

The Raman Spectrometer consists of three main units, an Argon ion laser (green), a Lieca LM optical microscope and a spectrometer for analyzing the wavelength of the emitted light which is collected using a CCD camera. Most of the light scattered from the specimen has the same frequency as the laser, however a tiny fraction experiences a frequency shift, which is characteristic of the chemical bonds or molecules present in the material. The Raman spectrum acquired using this instrument can give information on chemical composition, state, aggregation and even factors such as stress, orientation and temperature. Unlike FTIR, Raman spectroscopy looks at like-like bonds in a material (C-C, Si-Si etc). 48

Ramascope 2000

Raman Spectrometer

204

108

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<UL> <li>depth resolution 2um</li> <li> spatial resolution 1um </li><li> Argon ion laser (514.5nm - green)</li> </UL>

65.75

27.75

2009-06-03T15:43:54-05:00

2009-10-30T12:49:47-05:00

- 75

ScanArray Lite Microarray Analysis System

2009-10-30T12:49:47-05:00

2008-12-05T14:12:07-06:00

The Omicron VT-SPM is a UHV Scanning Probe Microscope that can operate both in scanning tunneling and atomic force modes. It can also operate at a range of temperatures down to liquid nitrogen. It was acquired, used, in 2006 and is operated by Prof Mike Trenery’s Surface Science Group. 13

VT-SPM

Scanning Probe Microscope

620

310

155

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195540

200

100

2009-07-13T12:30:59-05:00

2008-06-06T17:41:53-05:00

The Agilent 1946A LC-MSD single quadrupole LC-mass spectrometer is equipped with electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) sources. The LC system is an Agilent 1100 HPLC with refrigerated autoinjector and photodiode array detector for LC-UV-MS. This instrument is ideal for quantitative analysis and for profiling the constituents of complex mixtures. The Agilent 1946A LC-MSD was acquired in 1998 on a grant from Agilent to Dr. Richard B. van Breemen. 2

1946A LC-MSD

Single Quad LC Mass Spectrometer

inquire

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4318

Mass range is m/z 80 to m/z 2200 with unit mass resolution. Sensitivity is 10 ug reserpine in Selective Ion Monitoring (SIM) and 50 ug in scan mode.

2009-10-19T17:32:36-05:00

2008-12-05T13:39:08-06:00

The JEM-100CXII is a 100kV transmission electron microscope with a tungsten electron source. Because of its ease of operation this instrument is mainly used for teaching purposes, it is also used to check specimens before they are placed in the JEM-2010F or JEM-3010. The holder for this instrument is capable of taking two 3mm specimens. It was delivered in 1986 to Civil and Materials Engineering and was transferred to the RRC when the RRC-E facility opened in 1998. 9

JEM-100CX II

TEM

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<UL> <li>Resolution: 0.2nm lattice, 0.45nm point-to-point. </li><li>Accelerating Voltage: 20, 40, 60, 80, 100kV.</li><li>Magnification Range: 360 ~ 320,000x.</li> <li>Camera Length: 220~3,300mm.</li> <li>Tilt range: +/- 60 degrees.</li> <li>Specimen movement: 8.5mm (X), 2mm (Y), 1mm (Z)</li> </UL>

2009-06-03T15:43:28-05:00

2009-01-16T16:08:23-06:00

TBD 24

6410 QQQ

Triple Quad LC Mass Spectrometer

inquire

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Agilent6410QQQ.jpg

40922

TBD

2009-10-19T17:27:46-05:00

2009-01-16T12:45:32-06:00

The Thermo Finnigan TSQ Quantum triple quadrupole mass spectrometer is equipped with electrospray (ESI) and atmospheric pressure chemical ionization (APCI) sources. The mass spectrometer is capable of the following triple quad MS-MS experiments: Selective Reaction Monitoring (SRM), Multiple Reaction Monitoring (MRM), product ion scan, precusor ion scan, and neutral loss scan. The Surveyor HPLC system consists of a refrigerated auto sampler, gradient LC pumps, and photo diode array UV/vis absorbance detector for on-line LC-UV-MS-MS analyses. This is a highly sensitive and unusually high resolution triple quadrupole instrument that is ideal for quantitative analysis of compounds in complex mixtures. The Thermo Finnigan TSQ Quantum was installed in 2002 and purchased with internal funds. 20

TSQ Quantum

Triple Quad LC Mass Spectrometer

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4642

Mass range is a maximum of 4000 and resolution is 5000. The reserpine tuning solution has a concentration of 2 pg/uL.

2009-10-19T17:29:07-05:00

2009-07-13T11:54:28-05:00

The Ultrafast Physics Group in conjunction with RRC-E are developing an Ultrafast Electron Microscope. This instrument aims to combine the high spatial resolution of electron microscopy with the ultrafast (sub-picosecond) time resolution possible using ultrashort pulse laser systems. At present most work has centered on the development of the ultrafast electron source, eventually this will be mated to the bottom half of an existing TEM and made available to researchers at UIC. 66

UEM

Ultrafast Electron Microscope

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The aim of this project is to develop a functional prototype UEM with a space time resolution of less than 1nm-ps.

2009-07-14T15:02:17-05:00

2008-12-05T14:00:08-06:00

The Hitachi S-3000N is a Variable Pressure SEM and has a tungsten electron source. It was installed in 2000. It is capable of imaging specimens at high vacuum and also in a variable pressure range from 1-270 Pa. This allows non-conducting specimens to be imaged without coating with a conductive film. There is a secondary electron detector for use in high vacuum only, and backscattered and absorbed electron detectors, which can be used in both high vacuum and variable pressure modes. The microscope has an Oxford Inca EDX system with a light element X-ray detector (15mm WD). 11

S-3000N

Variable Pressure SEM

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<ul> <li>Accelerating Voltage 0.3-30kV</li> <li>Secondary electron image Resolution: 3.5nm (25kV, HV mode).</li> <li>Backscattered electron image Resolution: 5.0nm (25kV, VP mode).</li> <li>Magnification range: 15x ~ 300,000x.</li> <li>Variable pressure range: 1-270Pa.>/li> <li>Maximum Specimen size: 150mm diameter x 20mm high</li> <li>Specimen Tilt/ Rotation: 0-60 degrees, 360 degree rotation</li> <li>Specimen movement: 100mm (x), 50mm. (Y), 5~40mm (Z)</li> <li>Detectors: Secondary, Backscattered, Absorbed Current</li> <li>XEDS position: 15mm working disatnce, 35degree take off.</li> </ul>

2009-06-03T15:45:55-05:00

2009-06-25T09:19:12-05:00

Volocity 4.7 high-performance image analysis suite is a cross-platform modular program available to deal with more complex image analysis applications. Volocity is capable of deconvolving (removing haze from stacks) of images captured on either the confocal or widefield fluorescence systems. In addition, Volocity can render 3D- or 4D-models, track particle/organelle movement through stacks, and make automated measurements. Hosted on a four-processor Macintosh workstation. Software can be accessed on the RRC workstation or on individual lab computers using Keyserver web access. 60

Improvision

Volocity 4.7

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Volocity Visualization – View cells in 3D and 4D. Explore, rotate in real time. Provides high quality rendering with total interactivity. Volocity Quantitation – Find, measure, track and chart objects in your samples. Train Volocity to automatically identify the structure of interest, then ask it to find and measure objects in one time point, or track them over multiple time points. View the object tracks overlaid on the 2D images or 3D volume, to see the dynamics within your sample in a new way. Analyze your measurements by summarizing and extracting data from your results, and create charts in 2D or interactive 3D. Volocity Restoration –  Eliminate noise and blur in your data. Measured or calculated PSF generation options are included for confocal and wide field microscopes. The Fast Restoration tool provides an instantaneous improvement in XY resolution, or use Iterative Restoration for improvement in XY and Z resolution.

2009-07-02T10:17:37-05:00

2008-12-05T14:09:45-06:00

The Kratos AXIS-165 Surface Analysis System is a multi-technique instrument. Our instrument, installed in December 2004, is equipped for X-ray Photon Spectroscopy (XPS - also known as ESCA (Electron Spectroscopy for Chemical Analysis)) and Ion Scattering Spectroscopy (ISS). Both of these techniques are surface sensitive (less than 8nm for XPS and less than 1nm deep for ISS) with a spatial resolution in X and Y of down to 30 µm. For XPS the instrument has both a dual anode X-ray source (Al/Mg) and a Monochromatic Al X-ray source and is fitted with a charge neutralization coil. Depth profiling can be done either by tilting the specimen (non-destructive) or using an argon ion gun (destructive). Imaging XPS is possible by using scan plates to raster the focal point of the analyzer over the specimen. In ISS the low energy ions (1kV He) are scattered from only the top layer of atoms in the specimen and the energy of the backscattered ions is characteristic of the target atom. The energy spectrum obtained can be interpreted as a mass spectrum of the surface atoms. X-ray photoelectron spectroscopy is a surface-sensitive analytical technique that relies on the Photoelectric Effect discovered by Einstein (Nobel Prize 1921). It is a semi-quantitative method for obtaining composition and chemical state information. Because it is a surface-sensitive technique and the photoelectrons generally have low kinetic energies, XPS experiments must be conducted in an ultra-high vacuum (UHV) environment (10-9 – 10-10 Torr). The specimen is irradiated by X-rays of a fixed wavelength and the energies of photoelectrons generated are measured using a hemispherical analyzer. Given that the binding energy of each element’s orbitals is unique and is dependent on bonding environment, it can act as ‘fingerprints’ for identifying elements and chemical compounds. The photoelectrons that give rise to the spectra can only come from depths of less than 8 nm from the specimen surface. 12

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X-ray Photon Spectroscopy

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<UL> <li>Analyzer: 180degree, 165mm hemispherical analyzer</li> <li>Energy resolution < 0.45eV. </li> <li>X-ray sources: Dual anode (Al/Mg) achromatic, Monochromatic (Al). </li> <li>Charge neutralization: Kratos patented self compensating system. </li> <li>Heat/Cool facility: -100 degC to 600 degC in Prep Chamber. </li> <li>Vacuum: <5x10-10mb </li> </UL>

2009-06-19T15:21:24-05:00